c-Jun NH2-terminal kinase targeting and phosphorylation of heat shock factor-1 suppress its transcriptional activity

Rujuan Dai, Wojciech Frejtag, Bin He, Yan Zhang, Nahid F Mivechi

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Abstract

The mammalian heat shock transcription factor HSF-1 regulates the expression of the heat shock proteins, molecular chaperones that are involved in cellular processes from higher order assembly to protein degradation. HSF- 1 is a phosphorylated monomer under physiological growth conditions and is located mainly in the cytoplasm. Upon activation by a variety of environmental stresses, HSF-1 is translocated into the nucleus, forms trimers, acquires DNA binding activity, is hyperphosphorylated, appears as puncrate granules, and increases transcriptional activity of target genes. As cells recover from stress, the puncrate granules gradually disappear, and HSF-1 appears in a diffused staining pattern in the cytoplasm and nucleus. We have previously shown that the mitogen-activated protein kinase ERK phosphorylates and suppresses HSF-1-driven transcription. Here, we show that c-Jun NH2-terminal kinase (JNK) also phosphorylates and inactivates HSF-1. Overexpression of JNK facilitates the rapid disappearance of HSF-1 punctate granules after heat shock. Similar to ERK, JNK binds to HSF-1 in the conserved mitogenactivated protein kinases binding motifs and phosphorylates HSF-1 in the regulatory domain. The overexpression of an HSF-1-green fluorescent protein fusion construct lacking JNK phosphorylation sites causes this HSF-1 mutant to form nuclear granules that remain longer in the nucleus after heat shock. Taken together, these findings indicate that JNK phosphorylates HSF-1 and suppresses its transcriptional activity by rapidly clearing HSF-1 from the sites of transcription.

Original languageEnglish (US)
Pages (from-to)18210-18218
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number24
DOIs
StatePublished - Jun 16 2000

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Phosphorylation
JNK Mitogen-Activated Protein Kinases
Shock
Hot Temperature
Transcription
Cytoplasm
Molecular Chaperones
Heat-Shock Proteins
Green Fluorescent Proteins
Mitogen-Activated Protein Kinases
Protein Binding
Protein Kinases
Proteolysis
Fusion reactions
Genes
Monomers
Chemical activation
Staining and Labeling
Degradation
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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c-Jun NH2-terminal kinase targeting and phosphorylation of heat shock factor-1 suppress its transcriptional activity. / Dai, Rujuan; Frejtag, Wojciech; He, Bin; Zhang, Yan; Mivechi, Nahid F.

In: Journal of Biological Chemistry, Vol. 275, No. 24, 16.06.2000, p. 18210-18218.

Research output: Contribution to journalArticle

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