C-natriuretic peptide but not atrial natriuretic peptide increases cyclic GMP in cerebral arterial smooth muscle cells

Tao Hong, L. M. Zhang, Manuel R Castresana, S. D. Shillcutt, W. H. Newman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A-type natriuretic peptide (ANP) is found primarily in the heart and is released into the circulation. C-type (CNP) is found principally in the brain and has also been detected in the systemic circulation. When injected, both peptides produce vasodilatation most likely by elevation of guanosine 3′5′- cyclic monophosphate (cGMP) in smooth muscle cells via two distinct receptors, NPR-A and NPR-B. In this present study, we determined the effects of these two peptides on intracellular cGMP in smooth muscle cells cultured from pig cerebral and peripheral arteries. In smooth muscle cells cultured from the left anterior descending coronary artery, ANP and CNP increased cGMP with equal potency and efficacy (EC50 for ANP and CNP, 3.6 ± 0.2 × 10-8 M and 6.7 ± 0.8 × 10-8 M, respectively). In contrast, in smooth muscle cells from cerebral arteries, ANP was without effect while CNP increased cGMP in a concentration dependent manner ( EC50: 9.6 ± 1.7 × 10-8 M ). Stimulation of the soluble guanylyl cyclase with either nitroglycerin or nitroprusside was equivalent in the two cell types. The pattern of response of intracellular cGMP to CNP and ANP in isolated intact arteries from brain and heart was similar to that found in the cultured cells. These results suggest that smooth muscle cells in cerebral arteries express only NPR-B while cells from peripheral arteries can express both NPR-A and NPR-B.

Original languageEnglish (US)
Pages (from-to)2357-2365
Number of pages9
JournalLife Sciences
Volume56
Issue number26
DOIs
StatePublished - May 19 1995

Fingerprint

Natriuretic Peptides
Cyclic GMP
Atrial Natriuretic Factor
Smooth Muscle Myocytes
Muscle
Cells
Cerebral Arteries
Arteries
Brain
Peptides
Nitroglycerin
Nitroprusside
Vasodilation
Guanylate Cyclase
Cultured Cells
Coronary Vessels
Swine

Keywords

  • ANP
  • C-natriuretic peptide
  • cerebral arteries
  • peripheral arteries
  • vascular smooth muscle cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

C-natriuretic peptide but not atrial natriuretic peptide increases cyclic GMP in cerebral arterial smooth muscle cells. / Hong, Tao; Zhang, L. M.; Castresana, Manuel R; Shillcutt, S. D.; Newman, W. H.

In: Life Sciences, Vol. 56, No. 26, 19.05.1995, p. 2357-2365.

Research output: Contribution to journalArticle

Hong, Tao ; Zhang, L. M. ; Castresana, Manuel R ; Shillcutt, S. D. ; Newman, W. H. / C-natriuretic peptide but not atrial natriuretic peptide increases cyclic GMP in cerebral arterial smooth muscle cells. In: Life Sciences. 1995 ; Vol. 56, No. 26. pp. 2357-2365.
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