TY - JOUR
T1 - C. sakazakii activates AIM2 pathway accompanying with excessive ER stress response in mammalian mammary gland epithelium
AU - Song, Wenjuan
AU - Sheng, Le
AU - Chen, Fanghui
AU - Tian, Yu
AU - Li, Lian
AU - Wang, Genlin
AU - Li, Honglin
AU - Cai, Yafei
N1 - Publisher Copyright:
© 2020, Cell Stress Society International.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Bovine mastitis is a common inflammatory disease caused by various factors. The main factor of mastitis is pathogenic microorganism infection, such as Staphylococcus aureus, Escherichia coli, and Streptococcus. Cronobacter sakazakii (C. sakazakii) is a newly discovered pathogenic bacteria in milk products, which seriously threat human health in recent years. At present, it has not been reported that the pathogenesis of mastitis is caused by C. sakazakii. This study investigated the inflammation of mammary gland epithelium, which was induced by C. sakazakii for the first time. We focused on bacterial isolation, histological observation, AIM2 inflammasome pathways, endoplasmic reticulum stress, and apoptosis. The results showed that C. sakazakii–induced inflammation caused damage of tissue, significantly increased the production of pro-inflammatory cytokines (including TNF-α, IL-1β, and IL-6), activated the AIM2 inflammasome pathway (increased the expression of AIM2 and cleaved IL-1β), and induced endoplasmic reticulum stress (increased the expression of ERdj4, Chop, Grp78) and apoptosis (increased the ratio of Bax/Bcl-2, a marker of apoptosis). In conclusion, it is suggested that it maybe inhibite AIM2 inflammasome pathways and alleviate endoplasmic reticulum stress (ER stress) against the C. sakazakii–induced inflammation.
AB - Bovine mastitis is a common inflammatory disease caused by various factors. The main factor of mastitis is pathogenic microorganism infection, such as Staphylococcus aureus, Escherichia coli, and Streptococcus. Cronobacter sakazakii (C. sakazakii) is a newly discovered pathogenic bacteria in milk products, which seriously threat human health in recent years. At present, it has not been reported that the pathogenesis of mastitis is caused by C. sakazakii. This study investigated the inflammation of mammary gland epithelium, which was induced by C. sakazakii for the first time. We focused on bacterial isolation, histological observation, AIM2 inflammasome pathways, endoplasmic reticulum stress, and apoptosis. The results showed that C. sakazakii–induced inflammation caused damage of tissue, significantly increased the production of pro-inflammatory cytokines (including TNF-α, IL-1β, and IL-6), activated the AIM2 inflammasome pathway (increased the expression of AIM2 and cleaved IL-1β), and induced endoplasmic reticulum stress (increased the expression of ERdj4, Chop, Grp78) and apoptosis (increased the ratio of Bax/Bcl-2, a marker of apoptosis). In conclusion, it is suggested that it maybe inhibite AIM2 inflammasome pathways and alleviate endoplasmic reticulum stress (ER stress) against the C. sakazakii–induced inflammation.
KW - AIM2 inflammasome
KW - Apoptosis
KW - Cronobacter sakazakii
KW - ER stress
UR - http://www.scopus.com/inward/record.url?scp=85077682367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077682367&partnerID=8YFLogxK
U2 - 10.1007/s12192-019-01065-0
DO - 10.1007/s12192-019-01065-0
M3 - Article
C2 - 31925678
AN - SCOPUS:85077682367
SN - 1355-8145
VL - 25
SP - 223
EP - 233
JO - Cell Stress and Chaperones
JF - Cell Stress and Chaperones
IS - 2
ER -