C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss): Primary structure and vasorelaxant activities

Koji Inoue, Michael James Russell, Kenneth R. Olson, Yoshio Takei

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Natriuretic peptides (NPs) play important roles in osmoregulatory and cardiovascular systems of vertebrates. For functional studies of NPs, rainbow trout (Oncorhynchus mykiss), a euryhaline fish, is an interesting model. The information on homologous NPs of salmonid fish is, however, still incomplete with respect to C-type NP (CNP). In this study, we isolated cDNAs encoding the precursor of CNP from the brain of trout. Predicted mature CNP (CNP-22) sequence was identical to that of killifish Fundulus heteroclitus, and only one amino acid was different from that of the eel Anguilla japonica, demonstrating a greater conservation among different teleost species than is found with atrial NP (ANP) and ventricular NP (VNP). While the preprosegment of trout CNP retained 57% similarity to the eel sequence, similarities were low to those of sharks and tetrapods. The major site of expression identified by RT-PCR was the brain with minor expression in the atrium. The putative mature CNP-22 was synthesized and its biological activity was compared with other trout NPs (ANP and VNP) using trout ventral aorta, efferent branchial and celiacomesenteric arteries and anterior cardinal vein in vitro. Synthetic trout CNP-22 relaxed all pre-contracted vessels with potencies comparable to trout ANP and VNP.

Original languageEnglish (US)
Pages (from-to)185-192
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume130
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
natriuretic peptides
vasodilation
Trout
Oncorhynchus mykiss
Vasodilator Agents
trout
Natriuretic Peptides
Fundulidae
atrial natriuretic peptide
Eels
Atrial Natriuretic Factor
eel
Fishes
Anguilla
euryhaline species
brain
Sharks
Anguilla japonica
Fundulus heteroclitus

ASJC Scopus subject areas

  • Endocrinology

Cite this

C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : Primary structure and vasorelaxant activities. / Inoue, Koji; Russell, Michael James; Olson, Kenneth R.; Takei, Yoshio.

In: General and Comparative Endocrinology, Vol. 130, No. 2, 01.02.2003, p. 185-192.

Research output: Contribution to journalArticle

Inoue, Koji ; Russell, Michael James ; Olson, Kenneth R. ; Takei, Yoshio. / C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : Primary structure and vasorelaxant activities. In: General and Comparative Endocrinology. 2003 ; Vol. 130, No. 2. pp. 185-192.
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