Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med

C. D. Katsetos, M. M. Herman, L. Krishna, John R Vender, S. A. Vinores, D. P. Agamanolis, D. Schiffer, P. C. Burger, H. Urich

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective. - To evaluate the antigenic expression of calbindin-D(28k) in surgically resected cerebellar medulloblastomas and the human medulloblastoma cell line D283 Med in relation to glial neoplasms, the human glioblastoma (U- 251 MG) and rat glioma (C-6) cell lines, and other primary and metastatic brain tumors. Design. - Immunohistochemical staining was performed using an antiserum and a monoclonal antibody against calbindin-D(28k) on (1) formalin- fixed, paraffin-embedded human, predominantly posterior fossa, brain tumor specimens (49 medulloblastomas, 59 glial and mesenchymal primary central nervous system tumors, 1 posterior fossa rhabdoid tumor, and 34 metastatic tumors); (2) formalin-, 70% alcohol-, or Bouin's-fixed tumor cell lines (D283 Med, U-251 MG, and C-6) maintained in a three-dimensional gelatin foam (Gelfoam matrix) system, with or without treatment with dibutyryl cyclic adenosine monophosphate; and (3) formalin-fixed, paraffin-embedded C-6 glioma cells transplanted intracerebrally to rats. Results. - Calbindin-D(28k) immunohistochemical staining was detected in 20 of 49 cerebellar medulloblastomas and in cells of the human medulloblastoma cell line D283 Med grown in gelatin Gelfoam matrices, with or without treatment with dibutyryl cyclic adenosine monophosphate. In surgical resection specimens, calbindin- D(28k) reactivity was evident in populations of poorly differentiated cells of classic (non-nodular) medulloblastomas (16/20) and in mature Purkinje neuronlike phenotypes in medulloblastomas with ganglion cells (4/6) but was absent in desmoplastic medulloblastomas, including in areas of neoplastic neuritogenesis ('pale islands') (0/23). Calbindin-D(28k) staining was also present in D283 Med explants for up to 29 days in vitro. Reactivity was more widespread in dibutyryl cyclic adenosine monophosphate-treated cultures, coinciding with neuronal morphologic alterations of cultured cells. Focal calbindin-D(28k) staining was present in neural-like cells of an embryonal cerebellar tumor with divergent mesenchymal, epithelial, and neuroectodermal/neuroendocrine differentiation suggestive of a malignant rhabdoid tumor. No calbindin-D(28k) staining was obtained in primary glial and mesenchymal (intra- and extra-axial) brain tumors (0/59), in explants of human glioblastoma cell line U-251 MG, or in the rat glioma line C-6 maintained in Gelfoam matrices or transplanted intracerebrally. Among 34 epithelial and mesenchymal tumors metastatic to the posterior fossa, only subpopulations of cells in two small-cell (neuroendocrine) carcinomas originating in the lung were calbindin positive. Conclusions. - Calbindin- D(28k) expression in classic medulloblastomas, medulloblastomas with ganglion cells, and in the human medulloblastoma cell line D283 Med (which was derived from a metastatic classic medulloblastoma) suggests a phenotypic kinship between subsets of this tumor and neuronal progeny of the ventricular neuroepithelium, thus conferring additional support for its neuroblastic nature.

Original languageEnglish (US)
Pages (from-to)734-743
Number of pages10
JournalArchives of Pathology and Laboratory Medicine
Volume119
Issue number8
StatePublished - Jan 1 1995

Fingerprint

Calbindins
Medulloblastoma
Cell Line
Absorbable Gelatin Sponge
Staining and Labeling
Brain Neoplasms
Glioma
Neuroglia
Rhabdoid Tumor
Infratentorial Neoplasms
Cyclic AMP
Formaldehyde
Gelatin
Glioblastoma
Ganglia
Paraffin
Neoplasms
Cerebellar Neoplasms
Neuroendocrine Carcinoma
Central Nervous System Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Katsetos, C. D., Herman, M. M., Krishna, L., Vender, J. R., Vinores, S. A., Agamanolis, D. P., ... Urich, H. (1995). Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med. Archives of Pathology and Laboratory Medicine, 119(8), 734-743.

Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med. / Katsetos, C. D.; Herman, M. M.; Krishna, L.; Vender, John R; Vinores, S. A.; Agamanolis, D. P.; Schiffer, D.; Burger, P. C.; Urich, H.

In: Archives of Pathology and Laboratory Medicine, Vol. 119, No. 8, 01.01.1995, p. 734-743.

Research output: Contribution to journalArticle

Katsetos, CD, Herman, MM, Krishna, L, Vender, JR, Vinores, SA, Agamanolis, DP, Schiffer, D, Burger, PC & Urich, H 1995, 'Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med', Archives of Pathology and Laboratory Medicine, vol. 119, no. 8, pp. 734-743.
Katsetos, C. D. ; Herman, M. M. ; Krishna, L. ; Vender, John R ; Vinores, S. A. ; Agamanolis, D. P. ; Schiffer, D. ; Burger, P. C. ; Urich, H. / Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med. In: Archives of Pathology and Laboratory Medicine. 1995 ; Vol. 119, No. 8. pp. 734-743.
@article{e0d9eb246107443c98b145f114215c74,
title = "Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med",
abstract = "Objective. - To evaluate the antigenic expression of calbindin-D(28k) in surgically resected cerebellar medulloblastomas and the human medulloblastoma cell line D283 Med in relation to glial neoplasms, the human glioblastoma (U- 251 MG) and rat glioma (C-6) cell lines, and other primary and metastatic brain tumors. Design. - Immunohistochemical staining was performed using an antiserum and a monoclonal antibody against calbindin-D(28k) on (1) formalin- fixed, paraffin-embedded human, predominantly posterior fossa, brain tumor specimens (49 medulloblastomas, 59 glial and mesenchymal primary central nervous system tumors, 1 posterior fossa rhabdoid tumor, and 34 metastatic tumors); (2) formalin-, 70{\%} alcohol-, or Bouin's-fixed tumor cell lines (D283 Med, U-251 MG, and C-6) maintained in a three-dimensional gelatin foam (Gelfoam matrix) system, with or without treatment with dibutyryl cyclic adenosine monophosphate; and (3) formalin-fixed, paraffin-embedded C-6 glioma cells transplanted intracerebrally to rats. Results. - Calbindin-D(28k) immunohistochemical staining was detected in 20 of 49 cerebellar medulloblastomas and in cells of the human medulloblastoma cell line D283 Med grown in gelatin Gelfoam matrices, with or without treatment with dibutyryl cyclic adenosine monophosphate. In surgical resection specimens, calbindin- D(28k) reactivity was evident in populations of poorly differentiated cells of classic (non-nodular) medulloblastomas (16/20) and in mature Purkinje neuronlike phenotypes in medulloblastomas with ganglion cells (4/6) but was absent in desmoplastic medulloblastomas, including in areas of neoplastic neuritogenesis ('pale islands') (0/23). Calbindin-D(28k) staining was also present in D283 Med explants for up to 29 days in vitro. Reactivity was more widespread in dibutyryl cyclic adenosine monophosphate-treated cultures, coinciding with neuronal morphologic alterations of cultured cells. Focal calbindin-D(28k) staining was present in neural-like cells of an embryonal cerebellar tumor with divergent mesenchymal, epithelial, and neuroectodermal/neuroendocrine differentiation suggestive of a malignant rhabdoid tumor. No calbindin-D(28k) staining was obtained in primary glial and mesenchymal (intra- and extra-axial) brain tumors (0/59), in explants of human glioblastoma cell line U-251 MG, or in the rat glioma line C-6 maintained in Gelfoam matrices or transplanted intracerebrally. Among 34 epithelial and mesenchymal tumors metastatic to the posterior fossa, only subpopulations of cells in two small-cell (neuroendocrine) carcinomas originating in the lung were calbindin positive. Conclusions. - Calbindin- D(28k) expression in classic medulloblastomas, medulloblastomas with ganglion cells, and in the human medulloblastoma cell line D283 Med (which was derived from a metastatic classic medulloblastoma) suggests a phenotypic kinship between subsets of this tumor and neuronal progeny of the ventricular neuroepithelium, thus conferring additional support for its neuroblastic nature.",
author = "Katsetos, {C. D.} and Herman, {M. M.} and L. Krishna and Vender, {John R} and Vinores, {S. A.} and Agamanolis, {D. P.} and D. Schiffer and Burger, {P. C.} and H. Urich",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "119",
pages = "734--743",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "8",

}

TY - JOUR

T1 - Calbindin-D(28k) in subsets of medulloblastomas and in the human medulloblastoma cell line D283 med

AU - Katsetos, C. D.

AU - Herman, M. M.

AU - Krishna, L.

AU - Vender, John R

AU - Vinores, S. A.

AU - Agamanolis, D. P.

AU - Schiffer, D.

AU - Burger, P. C.

AU - Urich, H.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Objective. - To evaluate the antigenic expression of calbindin-D(28k) in surgically resected cerebellar medulloblastomas and the human medulloblastoma cell line D283 Med in relation to glial neoplasms, the human glioblastoma (U- 251 MG) and rat glioma (C-6) cell lines, and other primary and metastatic brain tumors. Design. - Immunohistochemical staining was performed using an antiserum and a monoclonal antibody against calbindin-D(28k) on (1) formalin- fixed, paraffin-embedded human, predominantly posterior fossa, brain tumor specimens (49 medulloblastomas, 59 glial and mesenchymal primary central nervous system tumors, 1 posterior fossa rhabdoid tumor, and 34 metastatic tumors); (2) formalin-, 70% alcohol-, or Bouin's-fixed tumor cell lines (D283 Med, U-251 MG, and C-6) maintained in a three-dimensional gelatin foam (Gelfoam matrix) system, with or without treatment with dibutyryl cyclic adenosine monophosphate; and (3) formalin-fixed, paraffin-embedded C-6 glioma cells transplanted intracerebrally to rats. Results. - Calbindin-D(28k) immunohistochemical staining was detected in 20 of 49 cerebellar medulloblastomas and in cells of the human medulloblastoma cell line D283 Med grown in gelatin Gelfoam matrices, with or without treatment with dibutyryl cyclic adenosine monophosphate. In surgical resection specimens, calbindin- D(28k) reactivity was evident in populations of poorly differentiated cells of classic (non-nodular) medulloblastomas (16/20) and in mature Purkinje neuronlike phenotypes in medulloblastomas with ganglion cells (4/6) but was absent in desmoplastic medulloblastomas, including in areas of neoplastic neuritogenesis ('pale islands') (0/23). Calbindin-D(28k) staining was also present in D283 Med explants for up to 29 days in vitro. Reactivity was more widespread in dibutyryl cyclic adenosine monophosphate-treated cultures, coinciding with neuronal morphologic alterations of cultured cells. Focal calbindin-D(28k) staining was present in neural-like cells of an embryonal cerebellar tumor with divergent mesenchymal, epithelial, and neuroectodermal/neuroendocrine differentiation suggestive of a malignant rhabdoid tumor. No calbindin-D(28k) staining was obtained in primary glial and mesenchymal (intra- and extra-axial) brain tumors (0/59), in explants of human glioblastoma cell line U-251 MG, or in the rat glioma line C-6 maintained in Gelfoam matrices or transplanted intracerebrally. Among 34 epithelial and mesenchymal tumors metastatic to the posterior fossa, only subpopulations of cells in two small-cell (neuroendocrine) carcinomas originating in the lung were calbindin positive. Conclusions. - Calbindin- D(28k) expression in classic medulloblastomas, medulloblastomas with ganglion cells, and in the human medulloblastoma cell line D283 Med (which was derived from a metastatic classic medulloblastoma) suggests a phenotypic kinship between subsets of this tumor and neuronal progeny of the ventricular neuroepithelium, thus conferring additional support for its neuroblastic nature.

AB - Objective. - To evaluate the antigenic expression of calbindin-D(28k) in surgically resected cerebellar medulloblastomas and the human medulloblastoma cell line D283 Med in relation to glial neoplasms, the human glioblastoma (U- 251 MG) and rat glioma (C-6) cell lines, and other primary and metastatic brain tumors. Design. - Immunohistochemical staining was performed using an antiserum and a monoclonal antibody against calbindin-D(28k) on (1) formalin- fixed, paraffin-embedded human, predominantly posterior fossa, brain tumor specimens (49 medulloblastomas, 59 glial and mesenchymal primary central nervous system tumors, 1 posterior fossa rhabdoid tumor, and 34 metastatic tumors); (2) formalin-, 70% alcohol-, or Bouin's-fixed tumor cell lines (D283 Med, U-251 MG, and C-6) maintained in a three-dimensional gelatin foam (Gelfoam matrix) system, with or without treatment with dibutyryl cyclic adenosine monophosphate; and (3) formalin-fixed, paraffin-embedded C-6 glioma cells transplanted intracerebrally to rats. Results. - Calbindin-D(28k) immunohistochemical staining was detected in 20 of 49 cerebellar medulloblastomas and in cells of the human medulloblastoma cell line D283 Med grown in gelatin Gelfoam matrices, with or without treatment with dibutyryl cyclic adenosine monophosphate. In surgical resection specimens, calbindin- D(28k) reactivity was evident in populations of poorly differentiated cells of classic (non-nodular) medulloblastomas (16/20) and in mature Purkinje neuronlike phenotypes in medulloblastomas with ganglion cells (4/6) but was absent in desmoplastic medulloblastomas, including in areas of neoplastic neuritogenesis ('pale islands') (0/23). Calbindin-D(28k) staining was also present in D283 Med explants for up to 29 days in vitro. Reactivity was more widespread in dibutyryl cyclic adenosine monophosphate-treated cultures, coinciding with neuronal morphologic alterations of cultured cells. Focal calbindin-D(28k) staining was present in neural-like cells of an embryonal cerebellar tumor with divergent mesenchymal, epithelial, and neuroectodermal/neuroendocrine differentiation suggestive of a malignant rhabdoid tumor. No calbindin-D(28k) staining was obtained in primary glial and mesenchymal (intra- and extra-axial) brain tumors (0/59), in explants of human glioblastoma cell line U-251 MG, or in the rat glioma line C-6 maintained in Gelfoam matrices or transplanted intracerebrally. Among 34 epithelial and mesenchymal tumors metastatic to the posterior fossa, only subpopulations of cells in two small-cell (neuroendocrine) carcinomas originating in the lung were calbindin positive. Conclusions. - Calbindin- D(28k) expression in classic medulloblastomas, medulloblastomas with ganglion cells, and in the human medulloblastoma cell line D283 Med (which was derived from a metastatic classic medulloblastoma) suggests a phenotypic kinship between subsets of this tumor and neuronal progeny of the ventricular neuroepithelium, thus conferring additional support for its neuroblastic nature.

UR - http://www.scopus.com/inward/record.url?scp=0028982954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028982954&partnerID=8YFLogxK

M3 - Article

C2 - 7646332

AN - SCOPUS:0028982954

VL - 119

SP - 734

EP - 743

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 8

ER -