Calcium activation in vascular smooth musclh by incubation of vessels in elevated fructose and glucose

X. C. Si, J. S. Jin, R Clinton Webb

Research output: Contribution to journalArticle

Abstract

A high fructose diet has been demonstrated to induce hypertension in seven animal models. We tested the acule effects of fructose and glucos incubations on vascular reactivity by measuring isometric force of aorti tissue in isolated tissue baths. Aortic rings (endothelium intact) fror Sprague-Dawley rats were incubated in physiological salt solution containin glucose (40 mM) or fructose (20 or 40 rnM) for 6 hours. Both treatments increased the contractile response to phenylephrine (10 -7 M) when compared to control vessels. A similar augmentation was observed in the contractile response to the calcium channel agonist (Bay K 8644) indicating calcium activation induced by fructose or glucose incubation. An agonist inducedsmooth muscle oscillation was also noted in elucose or fructose incubated vessels. The relaxation response 10 acelylcholine was not significantly altered in vessels incubated in fructose or glucose. We conclude tlKit calcium channel activation in vascular smooth muscle by iructose or glucose incubation may contribute to the high blood pressure in this model.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Fructose
Blood Vessels
Chemical activation
Calcium
Glucose
Muscle
Calcium Channel Agonists
Tissue
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
Hypertension
Blood pressure
Phenylephrine
Calcium Channels
Nutrition
Baths
Vascular Smooth Muscle
Endothelium
Sprague Dawley Rats
Rats
Animals

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Calcium activation in vascular smooth musclh by incubation of vessels in elevated fructose and glucose. / Si, X. C.; Jin, J. S.; Webb, R Clinton.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

@article{520787c23920480f88142e4a2d421cd4,
title = "Calcium activation in vascular smooth musclh by incubation of vessels in elevated fructose and glucose",
abstract = "A high fructose diet has been demonstrated to induce hypertension in seven animal models. We tested the acule effects of fructose and glucos incubations on vascular reactivity by measuring isometric force of aorti tissue in isolated tissue baths. Aortic rings (endothelium intact) fror Sprague-Dawley rats were incubated in physiological salt solution containin glucose (40 mM) or fructose (20 or 40 rnM) for 6 hours. Both treatments increased the contractile response to phenylephrine (10 -7 M) when compared to control vessels. A similar augmentation was observed in the contractile response to the calcium channel agonist (Bay K 8644) indicating calcium activation induced by fructose or glucose incubation. An agonist inducedsmooth muscle oscillation was also noted in elucose or fructose incubated vessels. The relaxation response 10 acelylcholine was not significantly altered in vessels incubated in fructose or glucose. We conclude tlKit calcium channel activation in vascular smooth muscle by iructose or glucose incubation may contribute to the high blood pressure in this model.",
author = "Si, {X. C.} and Jin, {J. S.} and Webb, {R Clinton}",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Calcium activation in vascular smooth musclh by incubation of vessels in elevated fructose and glucose

AU - Si, X. C.

AU - Jin, J. S.

AU - Webb, R Clinton

PY - 1996/12/1

Y1 - 1996/12/1

N2 - A high fructose diet has been demonstrated to induce hypertension in seven animal models. We tested the acule effects of fructose and glucos incubations on vascular reactivity by measuring isometric force of aorti tissue in isolated tissue baths. Aortic rings (endothelium intact) fror Sprague-Dawley rats were incubated in physiological salt solution containin glucose (40 mM) or fructose (20 or 40 rnM) for 6 hours. Both treatments increased the contractile response to phenylephrine (10 -7 M) when compared to control vessels. A similar augmentation was observed in the contractile response to the calcium channel agonist (Bay K 8644) indicating calcium activation induced by fructose or glucose incubation. An agonist inducedsmooth muscle oscillation was also noted in elucose or fructose incubated vessels. The relaxation response 10 acelylcholine was not significantly altered in vessels incubated in fructose or glucose. We conclude tlKit calcium channel activation in vascular smooth muscle by iructose or glucose incubation may contribute to the high blood pressure in this model.

AB - A high fructose diet has been demonstrated to induce hypertension in seven animal models. We tested the acule effects of fructose and glucos incubations on vascular reactivity by measuring isometric force of aorti tissue in isolated tissue baths. Aortic rings (endothelium intact) fror Sprague-Dawley rats were incubated in physiological salt solution containin glucose (40 mM) or fructose (20 or 40 rnM) for 6 hours. Both treatments increased the contractile response to phenylephrine (10 -7 M) when compared to control vessels. A similar augmentation was observed in the contractile response to the calcium channel agonist (Bay K 8644) indicating calcium activation induced by fructose or glucose incubation. An agonist inducedsmooth muscle oscillation was also noted in elucose or fructose incubated vessels. The relaxation response 10 acelylcholine was not significantly altered in vessels incubated in fructose or glucose. We conclude tlKit calcium channel activation in vascular smooth muscle by iructose or glucose incubation may contribute to the high blood pressure in this model.

UR - http://www.scopus.com/inward/record.url?scp=33749098292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749098292&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -