Calcium channel activation in arterioles from genetically hypertensive rats

Deborah S. Storm, Edward L. Stuenkel, R. Clinton Webb

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Enhanced contractile responsiveness to the calcium channel agonist Bay K 8644 has been documented in large conduit arteries and small muscular arteries from hypertensive rats. The present study examined the effects of Bay K 8644 on the intracellular calcium concentration ([Ca2+]i) in microvessels from stroke-prone spontaneously hypertensive rats and normotensive Wistar-Kyoto rats. Using microspectrofluorometry of fura-2, [Ca2+]i was measured in smooth muscle cells localized on arteriolar fragments (15-35 μm external diameter) isolated after collagenase digestion of the pancreas. Resting [Ca2+]i in hypertensive arterioles (94±6 nM, n=29) did not differ from that in normotensive vessels (81±4 nM, n=40). KCl (50 mM), applied alone and in the presence of Bay K 8644 (30 nM), stimulated increases in [Ca2+]i that were reversed in calcium-free solution and with nifedipine (10 μM), consistent with activation of potential-operated calcium channels. Potassium-induced calcium transients were consistently potentiated by Bay K 8644. The change in [Ca2+]i evoked by KCI alone or in combination with Bay K 8644 did not differ between arterioles from hypertensive and normotensive rats. In 24% of the vessels from hypertensive rats and in 29% of those from normotensive rats, Bay K 8644 evoked an increase in [Ca2+]i that did not differ significantly between the two strains. The findings indicate that, in contrast to observations made in larger arteries, there is no evidence of a functional abnormality in potentialoperated calcium channels in very small arterioles from genetically hypertensive rats.

Original languageEnglish (US)
Pages (from-to)380-388
Number of pages9
JournalHypertension
Volume20
Issue number3
DOIs
StatePublished - Sep 1992

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3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
Arterioles
Calcium Channels
Arteries
Calcium
Calcium Channel Agonists
Inbred WKY Rats
Fura-2
Collagenases
Inbred SHR Rats
Nifedipine
Microvessels
Smooth Muscle Myocytes
Pancreas
Digestion
Potassium
Stroke

Keywords

  • Arterioles
  • Bay K 8644
  • Calcium channels
  • Muscle, smooth, vascular
  • Nifedipine
  • Rats, inbred SHR

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Calcium channel activation in arterioles from genetically hypertensive rats. / Storm, Deborah S.; Stuenkel, Edward L.; Webb, R. Clinton.

In: Hypertension, Vol. 20, No. 3, 09.1992, p. 380-388.

Research output: Contribution to journalArticle

Storm, Deborah S. ; Stuenkel, Edward L. ; Webb, R. Clinton. / Calcium channel activation in arterioles from genetically hypertensive rats. In: Hypertension. 1992 ; Vol. 20, No. 3. pp. 380-388.
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