TY - JOUR
T1 - Calcium influx is required for endocytotic membrane retrieval
AU - Vogel, Steven S.
AU - Smith, Robert M.
AU - Baibakov, Boris
AU - Ikebuchi, Yoshihide
AU - Lambert, Nevin A
PY - 1999/4/27
Y1 - 1999/4/27
N2 - Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium- dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, ω-conotoxin MVIIC, ω- agatoxin IVA, and ω-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.
AB - Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium- dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, ω-conotoxin MVIIC, ω- agatoxin IVA, and ω-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.
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U2 - 10.1073/pnas.96.9.5019
DO - 10.1073/pnas.96.9.5019
M3 - Article
C2 - 10220411
AN - SCOPUS:0033609140
SN - 0027-8424
VL - 96
SP - 5019
EP - 5024
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -