Calcium influx is required for endocytotic membrane retrieval

Steven S. Vogel, Robert M. Smith, Boris Baibakov, Yoshihide Ikebuchi, Nevin A Lambert

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium- dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, ω-conotoxin MVIIC, ω- agatoxin IVA, and ω-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.

Original languageEnglish (US)
Pages (from-to)5019-5024
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number9
DOIs
StatePublished - Apr 27 1999

Fingerprint

Agatoxins
Calcium
Membranes
Cadmium
P-Type Calcium Channels
Conotoxins
Sea Urchins
Calcium Ionophores
Exocytosis
Fertilization
Membrane Potentials
Eggs

ASJC Scopus subject areas

  • General

Cite this

Calcium influx is required for endocytotic membrane retrieval. / Vogel, Steven S.; Smith, Robert M.; Baibakov, Boris; Ikebuchi, Yoshihide; Lambert, Nevin A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 9, 27.04.1999, p. 5019-5024.

Research output: Contribution to journalArticle

Vogel, Steven S. ; Smith, Robert M. ; Baibakov, Boris ; Ikebuchi, Yoshihide ; Lambert, Nevin A. / Calcium influx is required for endocytotic membrane retrieval. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 9. pp. 5019-5024.
@article{170c7ffe8aab4736819a47dce0fe1297,
title = "Calcium influx is required for endocytotic membrane retrieval",
abstract = "Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium- dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, ω-conotoxin MVIIC, ω- agatoxin IVA, and ω-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.",
author = "Vogel, {Steven S.} and Smith, {Robert M.} and Boris Baibakov and Yoshihide Ikebuchi and Lambert, {Nevin A}",
year = "1999",
month = "4",
day = "27",
doi = "10.1073/pnas.96.9.5019",
language = "English (US)",
volume = "96",
pages = "5019--5024",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "9",

}

TY - JOUR

T1 - Calcium influx is required for endocytotic membrane retrieval

AU - Vogel, Steven S.

AU - Smith, Robert M.

AU - Baibakov, Boris

AU - Ikebuchi, Yoshihide

AU - Lambert, Nevin A

PY - 1999/4/27

Y1 - 1999/4/27

N2 - Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium- dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, ω-conotoxin MVIIC, ω- agatoxin IVA, and ω-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.

AB - Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium- dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, ω-conotoxin MVIIC, ω- agatoxin IVA, and ω-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.

UR - http://www.scopus.com/inward/record.url?scp=0033609140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033609140&partnerID=8YFLogxK

U2 - 10.1073/pnas.96.9.5019

DO - 10.1073/pnas.96.9.5019

M3 - Article

C2 - 10220411

AN - SCOPUS:0033609140

VL - 96

SP - 5019

EP - 5024

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 9

ER -