Calcium preconditioning elicits strong protection against ischemic injury via protein kinase C signaling pathway

Hiroshi Miyawaki, Xiaobo Zhou, Muhammad Ashraf

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We tested the hypothesis that elevation of [Ca2+](i) during Ca2+ preconditioning (CPC) is a strong activator of protein kinase C (PKC) and confers unique protection against ischemic injury. CPC consisted of three cycles of Ca2+ depletion (1 minute each) and Ca2+ repletion (5 minutes each). Langendorff-perfused rat hearts were subjected to 40 minutes of global ischemia followed by 30 minutes of reperfusion. Significant functional recovery and decreased lactate dehydrogenase release were observed in CPC hearts compared with ischemic control hearts. In addition, ATP contents were significantly higher and cell structure was better preserved in CPC hearts than in ischemic control hearts. Administration of chelerythrine, a specific PKC inhibitor, completely abolished the CPC-induced cardioprotection. In other groups, in which Ca2+ influx during CPC was inhibited with verapamil, amiloride, and low Na+ perfusion, cardioprotection was significantly reduced. The prominent increase in the membrane PKC activity after CPC was in agreement with immunolocalization of PKC-α and PKC-δ in the cell membrane of CPC hearts. These results demonstrate that (1) a transient increase in [Ca2+](i) is a prominent feature of CPC and is a strong stimulus for the activation of PKC, (2) the elevation of [Ca2+](i) likely occurs via an L- type Ca2+ channel and Na+-Ca2+ exchanger, and (3) PKC plays a crucial role in the subcellular mechanisms of protection by CPC.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalCirculation research
Volume79
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Protein Kinase C
Calcium
Wounds and Injuries
Protein C Inhibitor
Amiloride
Protein Kinase Inhibitors
Verapamil
L-Lactate Dehydrogenase
Reperfusion
Membrane Proteins
Ischemia
Perfusion
Adenosine Triphosphate
Cell Membrane

Keywords

  • intracellular calcium
  • ischemia
  • preconditioning
  • protein kinase C

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Calcium preconditioning elicits strong protection against ischemic injury via protein kinase C signaling pathway. / Miyawaki, Hiroshi; Zhou, Xiaobo; Ashraf, Muhammad.

In: Circulation research, Vol. 79, No. 1, 01.01.1996, p. 137-146.

Research output: Contribution to journalArticle

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