Calcyon is necessary for activity-dependent AMPA receptor internalization and LTD in CA1 neurons of hippocampus

Heather Trantham Davidson, Jiping Xiao, Rujuan Dai, Clare M Bergson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Calcyon is a single transmembrane endocytic protein that regulates clathrin assembly and clathrin-mediated endocytosis in the brain. Ultrastructural studies indicate that calcyon localizes to spines, but whether it regulates glutamate neurotransmission is not known. Here, we show that deletion of the calcyon gene in mice inhibits agonist-stimulated endocytosis of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), without altering basal surface levels of the GluR1 or GluR2 subunits. Whole-cell patch-clamp studies of hippocampal neurons in culture and CA1 synapses in slices revealed that knockout (KO) of calcyon abolishes long-term synaptic depression (LTD), whereas mini-analysis in slices indicated basal transmission in the hippocampus is unaffected by the deletion. Further, transfection of green fluorescent protein-tagged calcyon rescued the ability of KO cultures to undergo LTD. In contrast, intracellular dialysis of a fusion protein containing the clathrin light-chain-binding domain of calcyon blocked the induction of LTD in wild-type hippocampal slices. Taken together, the present studies involving biochemical, immunological and electrophysiological analyses raise the possibility that calcyon plays a specialized role in regulating activity-dependent removal of synaptic AMPARs.

Original languageEnglish (US)
Pages (from-to)42-54
Number of pages13
JournalEuropean Journal of Neuroscience
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2009

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Long-Term Synaptic Depression
AMPA Receptors
Hippocampus
Neurons
Endocytosis
Clathrin Light Chains
Vesicular Transport Adaptor Proteins
Clathrin
Acids
Gene Deletion
Green Fluorescent Proteins
calcyon
Synaptic Transmission
Synapses
Transfection
Dialysis
Glutamic Acid
Spine
Brain

Keywords

  • ADHD
  • Clathrin-mediated endocytosis
  • Glutamate transmission
  • Schizophrenia
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Calcyon is necessary for activity-dependent AMPA receptor internalization and LTD in CA1 neurons of hippocampus. / Davidson, Heather Trantham; Xiao, Jiping; Dai, Rujuan; Bergson, Clare M.

In: European Journal of Neuroscience, Vol. 29, No. 1, 01.01.2009, p. 42-54.

Research output: Contribution to journalArticle

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