cAMP activates BK Ca channels in pulmonary arterial smooth muscle via cGMP-dependent protein kinase

Scott A Barman, Shu Zhu, Guichan Han, Richard E. White

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The signal transduction mechanisms defining the role of cyclic nucleotides in the regulation of pulmonary vascular tone is currently an area of great interest. Normally, signaling mechanisms that elevate cAMP and guanosine3′,5′-cyclic monophosphate (cGMP) maintain the pulmonary vasculature in a relaxed state. Modulation of the largeconductance, calcium- and voltage-activated potassium (BK Ca ) channel is important in the regulation of pulmonary arterial pressure, and inhibition (closing) of the BK Ca channel has been implicated in the development of pulmonary hypertension. Accordingly, studies were done to determine the effect of cAMP-elevating agents on BK Ca channel activity using patch-clamp studies in pulmonary arterial smooth muscle cells (PASMC) of the fawn-hooded rat (FHR), a recognized animal model of pulmonary hypertension. Forskolin (10 μM), a stimulator of adenylate cyclase and an activator of cAMP-dependent protein kinase (PKA), and 8-4-chlorophenylthio (CPT)-cAMP (100 μM), a membrane-permeable derivative of cAMP, opened BK Ca channels in single FHR PASMC. Treatment of FHR PASMC with 300 nM KT5823, a selective inhibitor of cGMP-dependent protein kinase (PKG) activity inhibited the effect of both forskolin and CPT-cAMP. In contrast, blocking PKA activation with 300 nM KT5720 had no effect on forskolin or CPT-cAMP-stimulated BK Ca channel activity. These results indicate that cAMP-dependent vasodilators activate BK Ca channels in PASMC of FHR via PKG-dependent and PKA-independent signaling pathways, which suggests cross-activation between cyclic nucleotide-dependent protein kinases in pulmonary arterial smooth muscle and therefore, a unique signaling pathway for cAMP-induced pulmonary vasodilation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume284
Issue number6 28-6
StatePublished - Jun 1 2003

Fingerprint

Large-Conductance Calcium-Activated Potassium Channels
Protein Kinases
Smooth Muscle
Lung
Smooth Muscle Myocytes
Colforsin
Cyclic Nucleotides
Pulmonary Hypertension
Cyclic AMP-Dependent Protein Kinases
Vasodilator Agents
Adenylyl Cyclases
Vasodilation
Blood Vessels
Signal Transduction
Potassium
Arterial Pressure
Animal Models
Calcium

Keywords

  • Cross-activation
  • High-conductance calcium-and voltage-activated potassium channel
  • cAMP-dependent protein kinase

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

cAMP activates BK Ca channels in pulmonary arterial smooth muscle via cGMP-dependent protein kinase . / Barman, Scott A; Zhu, Shu; Han, Guichan; White, Richard E.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 284, No. 6 28-6, 01.06.2003.

Research output: Contribution to journalArticle

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