Camptothecin-induced imbalance in intracellular cation homeostasis regulates programmed cell death in unicellular hemoflagellate Leishmania donovani

Nilkantha Sen, Benu Brata Das, Agneyo Ganguly, Tanmoy Mukherjee, Santu Bandyopadhyay, Hemanta K. Majumder

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Leishmania, a unicellular trypanosomatid protozoan parasite, causes a wide range of human diseases ranging from the localized self-healing cutaneous lesions to fatal visceral leishmaniasis. However, it undergoes a process of programmed cell death during treatment with the topoisomerase I poison camptothecin (CPT). The present study shows that CPT-induced formation of reactive oxygen species increases the level of cytosolic calcium through the release of calcium ions from intracellular stores as well as by influx of extracellular calcium. Elevation of cytosolic calcium is responsible for depolarization of mitochondrial membrane potential (ΔΨm), which is followed by a significant decrease in intracellular pH levels. CPT-induced oxidative stress also causes impairment of the Na+-K +-ATPase pump and subsequently decreases the intracellular K + level in leishmanial cells. A decrease in both intracellular pH and K+ levels propagates the apoptotic process through activation of caspase 3-like proteases by rapid formation of cytochrome c-mediated apoptotic complex. In addition to caspase-like protease activation, a lower level of intracellular K+ also enhances the activation of apoptotic nucleases at the late stage of apoptosis. This suggests that the physiological level of pH and K+ are inhibitory for apoptotic DNA fragmentation and caspase-like protease activation in leishmanial cells. Moreover, unlike mammalian cells, the intracellular ATP level gradually decreases with an increase in the number of apoptotic cells after the loss of ΔΨ m. Taken together, the elucidation of biochemical events, which tightly regulate the process of growth arrest and death of Leishmania donovani promastigotes, allows us to define a more comprehensive view of cell death during treatment with CPT.

Original languageEnglish (US)
Pages (from-to)52366-52375
Number of pages10
JournalJournal of Biological Chemistry
Volume279
Issue number50
DOIs
StatePublished - Dec 10 2004

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Leishmania donovani
Camptothecin
Cell death
Cations
Homeostasis
Cell Death
Chemical activation
Calcium
Peptide Hydrolases
Caspases
Cells
Type I DNA Topoisomerase
Oxidative stress
Visceral Leishmaniasis
Mitochondrial Membrane Potential
Poisons
Leishmania
Depolarization
DNA Fragmentation
Cytochromes c

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Camptothecin-induced imbalance in intracellular cation homeostasis regulates programmed cell death in unicellular hemoflagellate Leishmania donovani. / Sen, Nilkantha; Das, Benu Brata; Ganguly, Agneyo; Mukherjee, Tanmoy; Bandyopadhyay, Santu; Majumder, Hemanta K.

In: Journal of Biological Chemistry, Vol. 279, No. 50, 10.12.2004, p. 52366-52375.

Research output: Contribution to journalArticle

Sen, Nilkantha ; Das, Benu Brata ; Ganguly, Agneyo ; Mukherjee, Tanmoy ; Bandyopadhyay, Santu ; Majumder, Hemanta K. / Camptothecin-induced imbalance in intracellular cation homeostasis regulates programmed cell death in unicellular hemoflagellate Leishmania donovani. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 50. pp. 52366-52375.
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