Campylobacter jejuni CsrA regulates metabolic and virulence associated proteins and is necessary for mouse colonization

Joshua A. Fields, Jiaqi Li, Connor J. Gulbronson, David R. Hendrixson, Stuart A Thompson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Campylobacter jejuni infection is a leading bacterial cause of gastroenteritis and a common antecedent leading to Gullian-Barré syndrome. Our previous data suggested that the RNA-binding protein CsrA plays an important role in regulating several important phenotypes including motility, biofilm formation, and oxidative stress resistance. In this study, we compared the proteomes of wild type, csrA mutant, and complemented csrA mutant C. jejuni strains in an effort to elucidate the mechanisms by which CsrA affects virulence phenotypes. The putative CsrA regulon was more pronounced at stationary phase (111 regulated proteins) than at mid-log phase (25 regulated proteins). Proteins displaying altered expression in the csrA mutant included diverse metabolic functions, with roles in amino acid metabolism, TCA cycle, acetate metabolism, and various other cell processes, as well as pathogenesis-associated characteristics such as motility, chemotaxis, oxidative stress resistance, and fibronectin binding. The csrA mutant strain also showed altered autoagglutination kinetics when compared to the wild type. CsrA specifically bound the 5′ end of flaA mRNA, and we demonstrated that CsrA is a growth-phase dependent repressor of FlaA expression. Finally, the csrA mutant exhibited reduced ability to colonize in a mouse model when in competition with the wild type, further underscoring the role of CsrA in C. jejuni colonization and pathogenesis.

Original languageEnglish (US)
Article numbere0156932
JournalPloS one
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

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Campylobacter jejuni
Virulence
Oxidative stress
virulence
Metabolism
mutants
mice
Oxidative Stress
Campylobacter Infections
Phenotype
Regulon
Proteins
Aptitude
RNA-Binding Proteins
proteins
stress tolerance
Gastroenteritis
Biofilms
Chemotaxis
Proteome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Campylobacter jejuni CsrA regulates metabolic and virulence associated proteins and is necessary for mouse colonization. / Fields, Joshua A.; Li, Jiaqi; Gulbronson, Connor J.; Hendrixson, David R.; Thompson, Stuart A.

In: PloS one, Vol. 11, No. 6, e0156932, 01.06.2016.

Research output: Contribution to journalArticle

Fields, Joshua A. ; Li, Jiaqi ; Gulbronson, Connor J. ; Hendrixson, David R. ; Thompson, Stuart A. / Campylobacter jejuni CsrA regulates metabolic and virulence associated proteins and is necessary for mouse colonization. In: PloS one. 2016 ; Vol. 11, No. 6.
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