Cannabinoid receptors and their role in the regulation of the serotonin transporter in human placenta

Sean P. Kenney, Ramesh Kekuda, Puttur D Prasad, Frederick H. Leibach, Lawrence D Devoe, Vadivel Ganapathy

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

OBJECTIVE: We sought to investigate the expression of cannabinoid receptors in human placenta and BeWo choriocarcinoma cells and study their role in the regulation of the serotonin transporter. STUDY DESIGN: Expression of the 2 types of cannabinoid receptors (CB1 and CB2) in human placenta and BeWo cells was investigated by reverse transcriptase-polymerase chain reaction and Northern blot analysis. The involvement of the receptors in the regulation of the serotonin transporter expression was studied by using a cannabinoid receptor agonist (WIN 55212-2). BeWo cells were treated with the agonist in the presence or absence of forskolin, and the serotonin transporter activity was measured by assessing paroxetine-sensitive serotonin transport. Serotonin transporter density in cell membranes was monitored by measuring paroxetine-sensitive binding of RTI-55, a specific high-affinity ligand for the transporter. Agonist-induced changes in intracellular levels of cyclic adenosine monophosphate were also monitored. RESULTS: Reverse transcriptase-polymerase chain reaction and Northern blot analysis demonstrated unequivocally that human placenta and BeWo cells express both types of cannabinoid receptors. Treatment of BeWo cells with the receptor agonist blocked the activity of the constitutive, as well as the forskolin- induced, serotonin transporter without affecting the serotonin transporter density. This effect is not mediated by alterations in intracellular cyclic adenosine monophosphate levels. CONCLUSION: The results show that cannabinoid receptors are expressed in human placenta and BeWo cells and play a role in the regulation of the serotonin transporter activity. Human placenta is therefore a direct target for cannabinoids, and marijuana use during pregnancy is likely to affect the placental clearance of serotonin through the serotonin transporter.

Original languageEnglish (US)
Pages (from-to)491-497
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume181
Issue number2
DOIs
StatePublished - Jan 1 1999

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Cannabinoid Receptors
Serotonin Plasma Membrane Transport Proteins
Placenta
Paroxetine
Colforsin
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Cyclic AMP
Serotonin
Cannabinoid Receptor CB2
Cannabinoid Receptor Agonists
Cannabinoid Receptor CB1
Choriocarcinoma
Cannabinoids
Cannabis
Cell Membrane
Ligands
Pregnancy

Keywords

  • Cannabinoid receptors
  • Cyclic adenosine monophosphate
  • Marijuana
  • Placenta
  • Serotonin transporter

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Cannabinoid receptors and their role in the regulation of the serotonin transporter in human placenta. / Kenney, Sean P.; Kekuda, Ramesh; Prasad, Puttur D; Leibach, Frederick H.; Devoe, Lawrence D; Ganapathy, Vadivel.

In: American Journal of Obstetrics and Gynecology, Vol. 181, No. 2, 01.01.1999, p. 491-497.

Research output: Contribution to journalArticle

Kenney, Sean P. ; Kekuda, Ramesh ; Prasad, Puttur D ; Leibach, Frederick H. ; Devoe, Lawrence D ; Ganapathy, Vadivel. / Cannabinoid receptors and their role in the regulation of the serotonin transporter in human placenta. In: American Journal of Obstetrics and Gynecology. 1999 ; Vol. 181, No. 2. pp. 491-497.
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