Capillary density of skeletal muscle: A contributing mechanism for exercise intolerance in class II-III chronic heart failure independent of other peripheral alterations

Brian D. Duscha, William E. Kraus, Steven J. Keteyian, Martin J. Sullivan, Howard J. Green, Fred H. Schachat, Anne M. Pippen, Clinton A. Brawner, Jason M. Blank, Brian H. Annex

Research output: Contribution to journalArticle

Abstract

OBJECTIVES: The study was conducted to determine if the capillary density of skeletal muscle is a potential contributor to exercise intolerance in class II-III chronic heart failure (CHF). BACKGROUND: Previous studies suggest that abnormalities in skeletal muscle histology, contractile protein content and enzymology contribute to exercise intolerance in CHF. METHODS: The present study examined skeletal muscle biopsies from 22 male patients with CHF compared with 10 age-matched normal male control patients. Aerobic capacities, myosin heavy chain (MHC) isoforms, enzymes, and capillary density were measured. RESULTS: The patients with CHF demonstrated a reduced peak oxygen consumption when compared to controls (15.0 ± 2.5 vs. 19.8 ± 5.0 ml · kg-1 · min-1, p <0.05). Using cell-specific antibodies to directly assess vascular density, there was a reduction in capillary density in CHF measured as the number of endothelial cells/fiber (1.42 ± 0.28 vs. 1.74 ± 0.35, p = 0.02). In CHF, capillary density was inversely related to maximal oxygen consumption (r = 0.479, p = 0.02). The MHC IIx isoform was found to be higher in patients with CHF versus normal subjects (28.5 ± 13.6 vs. 19.5 ± 9.4, p <0.05). CONCLUSIONS: There was a significant reduction in microvascular density in patients with CHF compared with the control group, without major differences in other usual histologic and biochemical aerobic markers. The inverse relationship with peak oxygen consumption seen in the CHF group suggests that a reduction in microvascular density of skeletal muscle may precede other skeletal muscle alterations and play a critical role in the exercise intolerance characteristic of patients with CHF.

Original languageEnglish (US)
Pages (from-to)1956-1963
Number of pages8
JournalJournal of the American College of Cardiology
Volume33
Issue number7
DOIs
StatePublished - Jun 1 1999
Externally publishedYes

Fingerprint

Skeletal Muscle
Heart Failure
Exercise
Oxygen Consumption
Myosin Heavy Chains
Protein Isoforms
Contractile Proteins
Blood Vessels
Histology
Endothelial Cells
Biomarkers
Biopsy
Control Groups
Antibodies
Enzymes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Capillary density of skeletal muscle : A contributing mechanism for exercise intolerance in class II-III chronic heart failure independent of other peripheral alterations. / Duscha, Brian D.; Kraus, William E.; Keteyian, Steven J.; Sullivan, Martin J.; Green, Howard J.; Schachat, Fred H.; Pippen, Anne M.; Brawner, Clinton A.; Blank, Jason M.; Annex, Brian H.

In: Journal of the American College of Cardiology, Vol. 33, No. 7, 01.06.1999, p. 1956-1963.

Research output: Contribution to journalArticle

Duscha, Brian D. ; Kraus, William E. ; Keteyian, Steven J. ; Sullivan, Martin J. ; Green, Howard J. ; Schachat, Fred H. ; Pippen, Anne M. ; Brawner, Clinton A. ; Blank, Jason M. ; Annex, Brian H. / Capillary density of skeletal muscle : A contributing mechanism for exercise intolerance in class II-III chronic heart failure independent of other peripheral alterations. In: Journal of the American College of Cardiology. 1999 ; Vol. 33, No. 7. pp. 1956-1963.
@article{d0f94f3a5c3a45ab8438190e711a2d11,
title = "Capillary density of skeletal muscle: A contributing mechanism for exercise intolerance in class II-III chronic heart failure independent of other peripheral alterations",
abstract = "OBJECTIVES: The study was conducted to determine if the capillary density of skeletal muscle is a potential contributor to exercise intolerance in class II-III chronic heart failure (CHF). BACKGROUND: Previous studies suggest that abnormalities in skeletal muscle histology, contractile protein content and enzymology contribute to exercise intolerance in CHF. METHODS: The present study examined skeletal muscle biopsies from 22 male patients with CHF compared with 10 age-matched normal male control patients. Aerobic capacities, myosin heavy chain (MHC) isoforms, enzymes, and capillary density were measured. RESULTS: The patients with CHF demonstrated a reduced peak oxygen consumption when compared to controls (15.0 ± 2.5 vs. 19.8 ± 5.0 ml · kg-1 · min-1, p <0.05). Using cell-specific antibodies to directly assess vascular density, there was a reduction in capillary density in CHF measured as the number of endothelial cells/fiber (1.42 ± 0.28 vs. 1.74 ± 0.35, p = 0.02). In CHF, capillary density was inversely related to maximal oxygen consumption (r = 0.479, p = 0.02). The MHC IIx isoform was found to be higher in patients with CHF versus normal subjects (28.5 ± 13.6 vs. 19.5 ± 9.4, p <0.05). CONCLUSIONS: There was a significant reduction in microvascular density in patients with CHF compared with the control group, without major differences in other usual histologic and biochemical aerobic markers. The inverse relationship with peak oxygen consumption seen in the CHF group suggests that a reduction in microvascular density of skeletal muscle may precede other skeletal muscle alterations and play a critical role in the exercise intolerance characteristic of patients with CHF.",
author = "Duscha, {Brian D.} and Kraus, {William E.} and Keteyian, {Steven J.} and Sullivan, {Martin J.} and Green, {Howard J.} and Schachat, {Fred H.} and Pippen, {Anne M.} and Brawner, {Clinton A.} and Blank, {Jason M.} and Annex, {Brian H.}",
year = "1999",
month = "6",
day = "1",
doi = "10.1016/S0735-1097(99)00101-1",
language = "English (US)",
volume = "33",
pages = "1956--1963",
journal = "Journal of the American College of Cardiology",
issn = "0735-1097",
publisher = "Elsevier USA",
number = "7",

}

TY - JOUR

T1 - Capillary density of skeletal muscle

T2 - A contributing mechanism for exercise intolerance in class II-III chronic heart failure independent of other peripheral alterations

AU - Duscha, Brian D.

AU - Kraus, William E.

AU - Keteyian, Steven J.

AU - Sullivan, Martin J.

AU - Green, Howard J.

AU - Schachat, Fred H.

AU - Pippen, Anne M.

AU - Brawner, Clinton A.

AU - Blank, Jason M.

AU - Annex, Brian H.

PY - 1999/6/1

Y1 - 1999/6/1

N2 - OBJECTIVES: The study was conducted to determine if the capillary density of skeletal muscle is a potential contributor to exercise intolerance in class II-III chronic heart failure (CHF). BACKGROUND: Previous studies suggest that abnormalities in skeletal muscle histology, contractile protein content and enzymology contribute to exercise intolerance in CHF. METHODS: The present study examined skeletal muscle biopsies from 22 male patients with CHF compared with 10 age-matched normal male control patients. Aerobic capacities, myosin heavy chain (MHC) isoforms, enzymes, and capillary density were measured. RESULTS: The patients with CHF demonstrated a reduced peak oxygen consumption when compared to controls (15.0 ± 2.5 vs. 19.8 ± 5.0 ml · kg-1 · min-1, p <0.05). Using cell-specific antibodies to directly assess vascular density, there was a reduction in capillary density in CHF measured as the number of endothelial cells/fiber (1.42 ± 0.28 vs. 1.74 ± 0.35, p = 0.02). In CHF, capillary density was inversely related to maximal oxygen consumption (r = 0.479, p = 0.02). The MHC IIx isoform was found to be higher in patients with CHF versus normal subjects (28.5 ± 13.6 vs. 19.5 ± 9.4, p <0.05). CONCLUSIONS: There was a significant reduction in microvascular density in patients with CHF compared with the control group, without major differences in other usual histologic and biochemical aerobic markers. The inverse relationship with peak oxygen consumption seen in the CHF group suggests that a reduction in microvascular density of skeletal muscle may precede other skeletal muscle alterations and play a critical role in the exercise intolerance characteristic of patients with CHF.

AB - OBJECTIVES: The study was conducted to determine if the capillary density of skeletal muscle is a potential contributor to exercise intolerance in class II-III chronic heart failure (CHF). BACKGROUND: Previous studies suggest that abnormalities in skeletal muscle histology, contractile protein content and enzymology contribute to exercise intolerance in CHF. METHODS: The present study examined skeletal muscle biopsies from 22 male patients with CHF compared with 10 age-matched normal male control patients. Aerobic capacities, myosin heavy chain (MHC) isoforms, enzymes, and capillary density were measured. RESULTS: The patients with CHF demonstrated a reduced peak oxygen consumption when compared to controls (15.0 ± 2.5 vs. 19.8 ± 5.0 ml · kg-1 · min-1, p <0.05). Using cell-specific antibodies to directly assess vascular density, there was a reduction in capillary density in CHF measured as the number of endothelial cells/fiber (1.42 ± 0.28 vs. 1.74 ± 0.35, p = 0.02). In CHF, capillary density was inversely related to maximal oxygen consumption (r = 0.479, p = 0.02). The MHC IIx isoform was found to be higher in patients with CHF versus normal subjects (28.5 ± 13.6 vs. 19.5 ± 9.4, p <0.05). CONCLUSIONS: There was a significant reduction in microvascular density in patients with CHF compared with the control group, without major differences in other usual histologic and biochemical aerobic markers. The inverse relationship with peak oxygen consumption seen in the CHF group suggests that a reduction in microvascular density of skeletal muscle may precede other skeletal muscle alterations and play a critical role in the exercise intolerance characteristic of patients with CHF.

UR - http://www.scopus.com/inward/record.url?scp=0032720717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032720717&partnerID=8YFLogxK

U2 - 10.1016/S0735-1097(99)00101-1

DO - 10.1016/S0735-1097(99)00101-1

M3 - Article

C2 - 10362199

AN - SCOPUS:0032720717

VL - 33

SP - 1956

EP - 1963

JO - Journal of the American College of Cardiology

JF - Journal of the American College of Cardiology

SN - 0735-1097

IS - 7

ER -