Capillary electrophoresis determination of biflavanones from Garcinia kola in three traditional African medicinal formulations

Chris O. Okunji, Tantalia A. Ware, Rickey P. Hicks, Maurice M. Iwu, David J. Skanchy

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A rapid capillary electrophoresis (CE) method for the quantification of four biologically active biflavanones present in three different traditional African medicinal preparations from the seeds of Garcinia kola was developed. The four biflavanones of interest (GB1, GB2 and GB1-glycoside and kolaflavanone) were quantified in a traditional tea preparation, and two commercially available ethanolic formulations. The optimum separation conditions consisted of a 100 mM borate, pH 9.5 running buffer, which gave baseline resolution of all four components in less than 12 minutes. Linear calibration ranges for each component were between 2.5 and 1000 μg/mL. Limits of detection for the biflavanones quantified in this study were between 3 and 6 μg/mL. The "fingerprint" of the biflavanones in the aqueous tea and two ethanolic formulations was found to be similar, however concentrations of the four biflavanones were up to 50 fold higher in the ethanolic preparations. The major component in all three formulations was GB1.

Original languageEnglish (US)
Pages (from-to)440-444
Number of pages5
JournalPlanta Medica
Volume68
Issue number5
DOIs
StatePublished - Jun 19 2002

    Fingerprint

Keywords

  • Biflavanone
  • Capillary electrophoresis
  • Garcinia kola
  • Kolaviron

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this