Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro

A. Tezvergil-Mutluay, M. M. Mutluay, K. A. Agee, R. Seseogullari-Dirihan, T. Hoshika, M. Cadenaro, L. Breschi, P. Vallittu, F. R. Tay, D. H. Pashley

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) cause collagen degradation in hybrid layers created by dentin adhesives. This in vitro study evaluated the feasibility of using a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), to inactivate soluble rhMMP-9, as an example of dentin MMPs, and matrix-bound dentin proteases. The inhibitory effects of 5 EDC concentrations (0.01-0.3 M) and 5 incubation times (1-30 min) on soluble rhMMP-9 were screened with an MMP assay kit. The same EDC concentrations were used to evaluate their inhibitory effects on endogenous proteinases from completely demineralized dentin beams that were incubated in simulated body fluid for 30 days. Decreases in modulus of elasticity (E) and dry mass of the beams, and increases in hydroxyproline content of hydrolysates derived from the incubation medium were used as indirect measures of matrix collagen hydrolysis. All EDC concentrations and pre-treatment times inactivated MMP-9 by 98% to 100% (p < 0.05) compared with non-cross-linked controls. Dentin beams incubated in 0.3 M EDC showed only a 9% decrease in E (45% decrease in control), a 3.6% to 5% loss of dry mass (18% loss in control), and significantly less solubilized hydroxyproline when compared with the control without EDC cross-linking (p < 0.05). It is concluded that EDC application for 1 min may be a clinically relevant and effective means for inactivating soluble rhMMP-9 and matrix-bound dentin proteinases if further studies demonstrate that EDC is not toxic to pulpal tissues.

Original languageEnglish (US)
Pages (from-to)192-196
Number of pages5
JournalJournal of Dental Research
Volume91
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

Carbodiimides
Dentin
Matrix Metalloproteinases
Peptide Hydrolases
Hydroxyproline
Collagen
Ethyldimethylaminopropyl Carbodiimide
Elastic Modulus
Poisons
Matrix Metalloproteinase 9
Feasibility Studies
Body Fluids
Adhesives
In Vitro Techniques
Hydrolysis

Keywords

  • carbodiimide
  • degradation
  • dentin
  • hydroxyproline
  • matrix metalloproteinase
  • soluble collagen

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tezvergil-Mutluay, A., Mutluay, M. M., Agee, K. A., Seseogullari-Dirihan, R., Hoshika, T., Cadenaro, M., ... Pashley, D. H. (2012). Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro. Journal of Dental Research, 91(2), 192-196. https://doi.org/10.1177/0022034511427705

Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro. / Tezvergil-Mutluay, A.; Mutluay, M. M.; Agee, K. A.; Seseogullari-Dirihan, R.; Hoshika, T.; Cadenaro, M.; Breschi, L.; Vallittu, P.; Tay, F. R.; Pashley, D. H.

In: Journal of Dental Research, Vol. 91, No. 2, 01.02.2012, p. 192-196.

Research output: Contribution to journalArticle

Tezvergil-Mutluay, A, Mutluay, MM, Agee, KA, Seseogullari-Dirihan, R, Hoshika, T, Cadenaro, M, Breschi, L, Vallittu, P, Tay, FR & Pashley, DH 2012, 'Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro', Journal of Dental Research, vol. 91, no. 2, pp. 192-196. https://doi.org/10.1177/0022034511427705
Tezvergil-Mutluay A, Mutluay MM, Agee KA, Seseogullari-Dirihan R, Hoshika T, Cadenaro M et al. Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro. Journal of Dental Research. 2012 Feb 1;91(2):192-196. https://doi.org/10.1177/0022034511427705
Tezvergil-Mutluay, A. ; Mutluay, M. M. ; Agee, K. A. ; Seseogullari-Dirihan, R. ; Hoshika, T. ; Cadenaro, M. ; Breschi, L. ; Vallittu, P. ; Tay, F. R. ; Pashley, D. H. / Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro. In: Journal of Dental Research. 2012 ; Vol. 91, No. 2. pp. 192-196.
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