Carbodiimide inactivation of MMPs and effect on dentin bonding

A. Mazzoni, F. M. Apolonio, V. P A Saboia, S. Santi, V. Angeloni, V. Checchi, R. Curci, R. Di Lenarda, Franklin Chi Meng Tay, D. H. Pashley, L. Breschi

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy showed that hybrid layers of tested adhesives exhibited intense collagenolytic activity, while almost no fluorescence signal was detected when specimens were pre-treated with EDC. The correlative analysis used in this study demonstrated that EDC could contribute to inactivate endogenous dentin MMPs within the hybrid layer created by etch-and-rinse adhesives.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
JournalJournal of Dental Research
Volume93
Issue number3
DOIs
StatePublished - Mar 1 2014

Fingerprint

Carbodiimides
Dentin
Matrix Metalloproteinases
Adhesives
Ethyldimethylaminopropyl Carbodiimide
Gelatinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Powders
Proteins
Fluorescence
Acids

Keywords

  • adhesive systems
  • biochemical assays
  • collagen cross-linker
  • dentin bonding agents
  • endogenous proteinases
  • human dentin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Mazzoni, A., Apolonio, F. M., Saboia, V. P. A., Santi, S., Angeloni, V., Checchi, V., ... Breschi, L. (2014). Carbodiimide inactivation of MMPs and effect on dentin bonding. Journal of Dental Research, 93(3), 263-268. https://doi.org/10.1177/0022034513516465

Carbodiimide inactivation of MMPs and effect on dentin bonding. / Mazzoni, A.; Apolonio, F. M.; Saboia, V. P A; Santi, S.; Angeloni, V.; Checchi, V.; Curci, R.; Di Lenarda, R.; Tay, Franklin Chi Meng; Pashley, D. H.; Breschi, L.

In: Journal of Dental Research, Vol. 93, No. 3, 01.03.2014, p. 263-268.

Research output: Contribution to journalArticle

Mazzoni, A, Apolonio, FM, Saboia, VPA, Santi, S, Angeloni, V, Checchi, V, Curci, R, Di Lenarda, R, Tay, FCM, Pashley, DH & Breschi, L 2014, 'Carbodiimide inactivation of MMPs and effect on dentin bonding', Journal of Dental Research, vol. 93, no. 3, pp. 263-268. https://doi.org/10.1177/0022034513516465
Mazzoni A, Apolonio FM, Saboia VPA, Santi S, Angeloni V, Checchi V et al. Carbodiimide inactivation of MMPs and effect on dentin bonding. Journal of Dental Research. 2014 Mar 1;93(3):263-268. https://doi.org/10.1177/0022034513516465
Mazzoni, A. ; Apolonio, F. M. ; Saboia, V. P A ; Santi, S. ; Angeloni, V. ; Checchi, V. ; Curci, R. ; Di Lenarda, R. ; Tay, Franklin Chi Meng ; Pashley, D. H. ; Breschi, L. / Carbodiimide inactivation of MMPs and effect on dentin bonding. In: Journal of Dental Research. 2014 ; Vol. 93, No. 3. pp. 263-268.
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