Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury

Lijuan Chen, Yingjie Wang, Yaohua Pan, Lan Zhang, Chengxing Shen, Gangjian Qin, Muhammad Ashraf, Neal Weintraub, Genshan Ma, Yaoliang Tang

Research output: Contribution to journalArticle

190 Scopus citations

Abstract

Background: Cardiac progenitors (CPC) mediate cardioprotection via paracrine effects. To date, most of studies focused on secreted paracrine proteins. Here we investigated the CPC-derived-exosomes on protecting myocardium from acute ischemia/reperfusion (MI/R) injury. Methods and results: CPC were isolated from mouse heart using two-step protocol. Exosomes were purified from conditional medium, and confirmed by electron micrograph and Western blot using CD63 as a marker. qRT-PCR shows that CPC-exosomes have high level expression of GATA4-responsive-miR-451. Exosomes were ex vivo labeled with PKH26, We observed exosomes can be uptaken by H9C2 cardiomyoblasts with high efficiency after 12. h incubation. CPC-exosomes protect H9C2 from oxidative stress by inhibiting caspase 3/7 activation in vitro. In vivo delivery of CPC-exosomes in an acute mouse myocardial ischemia/reperfusion model inhibited cardiomyocyte apoptosis by about 53% in comparison with PBS control (. p<. 0.05). Conclusion: Our results suggest, for the first time, the CPC-exosomes can be used as a therapeutic vehicle for cardioprotection, and highlights a new perspective for using non-cell exosomes for cardiac disease.

Original languageEnglish (US)
Pages (from-to)566-571
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume431
Issue number3
DOIs
Publication statusPublished - Feb 15 2013
Externally publishedYes

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Keywords

  • Apoptosis
  • Cardiac progenitors
  • Exosomes
  • Ischemia/reperfusion
  • MicroRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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