Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury

Lijuan Chen, Yingjie Wang, Yaohua Pan, Lan Zhang, Chengxing Shen, Gangjian Qin, Muhammad Ashraf, Neal Lee Weintraub, Genshan Ma, Yao Liang Tang

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Background: Cardiac progenitors (CPC) mediate cardioprotection via paracrine effects. To date, most of studies focused on secreted paracrine proteins. Here we investigated the CPC-derived-exosomes on protecting myocardium from acute ischemia/reperfusion (MI/R) injury. Methods and results: CPC were isolated from mouse heart using two-step protocol. Exosomes were purified from conditional medium, and confirmed by electron micrograph and Western blot using CD63 as a marker. qRT-PCR shows that CPC-exosomes have high level expression of GATA4-responsive-miR-451. Exosomes were ex vivo labeled with PKH26, We observed exosomes can be uptaken by H9C2 cardiomyoblasts with high efficiency after 12. h incubation. CPC-exosomes protect H9C2 from oxidative stress by inhibiting caspase 3/7 activation in vitro. In vivo delivery of CPC-exosomes in an acute mouse myocardial ischemia/reperfusion model inhibited cardiomyocyte apoptosis by about 53% in comparison with PBS control (. p<. 0.05). Conclusion: Our results suggest, for the first time, the CPC-exosomes can be used as a therapeutic vehicle for cardioprotection, and highlights a new perspective for using non-cell exosomes for cardiac disease.

Original languageEnglish (US)
Pages (from-to)566-571
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume431
Issue number3
DOIs
StatePublished - Feb 15 2013
Externally publishedYes

Fingerprint

Exosomes
Oxidative stress
Reperfusion Injury
Caspase 3
Myocardium
Chemical activation
Apoptosis
Electrons
Proteins
Caspase 7
PKH 26
Myocardial Reperfusion
Cardiac Myocytes
Myocardial Ischemia
Heart Diseases
Oxidative Stress
Western Blotting
Polymerase Chain Reaction

Keywords

  • Apoptosis
  • Cardiac progenitors
  • Exosomes
  • Ischemia/reperfusion
  • MicroRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury. / Chen, Lijuan; Wang, Yingjie; Pan, Yaohua; Zhang, Lan; Shen, Chengxing; Qin, Gangjian; Ashraf, Muhammad; Weintraub, Neal Lee; Ma, Genshan; Tang, Yao Liang.

In: Biochemical and Biophysical Research Communications, Vol. 431, No. 3, 15.02.2013, p. 566-571.

Research output: Contribution to journalArticle

Chen, Lijuan ; Wang, Yingjie ; Pan, Yaohua ; Zhang, Lan ; Shen, Chengxing ; Qin, Gangjian ; Ashraf, Muhammad ; Weintraub, Neal Lee ; Ma, Genshan ; Tang, Yao Liang. / Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 431, No. 3. pp. 566-571.
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