Cardiotonic agents: A simple HPLC procedure for the quantitation of dobutamine and a new congener in plasma

R. Dixon, J. Hsiao, W. Caldwell

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A simple high performance-liquid chromatographic procedure employing electrochemical detection (LC-EC) has been developed for the quantitation of the cardiotonic agent dobutamine and a new pharmacologically active congener. The drugs were extracted from plasma by adsorption on to alumina, eluted from the adsorbent with dilute perchloric acid and the eluate subjected to LC-EC. The method had a limit of sensitivity of 1 ng/ml of plasma for each drug. The applicability of the LC-EC procedure was demonstrated by determining steady-state plasma concentration profiles of dobutamine and its congener in man and dog, respectively, following i.v. infusion of the drugs.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume48
Issue number2
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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