Ca2+ and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal

Xin Sheng Wu, Benjamin D. McNeil, Jianhua Xu, Junmei Fan, Lei Xue, Ernestina Melicoff, Roberto Adachi, Li Bai, Ling Gang Wu

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Although endocytosis maintains synaptic transmission, how endocytosis is initiated is unclear. We found that calcium influx initiated all forms of endocytosis at a single nerve terminal in rodents, including clathrin-dependent slow endocytosis, bulk endocytosis, rapid endocytosis and endocytosis overshoot (excess endocytosis), with each being evoked with a correspondingly higher calcium threshold. As calcium influx increased, endocytosis gradually switched from very slow endocytosis to slow endocytosis to bulk endocytosis to rapid endocytosis and to endocytosis overshoot. The calcium-induced endocytosis rate increase was a result of the speeding up of membrane invagination and fission. Pharmacological experiments suggested that the calcium sensor mediating these forms of endocytosis is calmodulin. In addition to its role in recycling vesicles, calcium/calmodulin-initiated endocytosis facilitated vesicle mobilization to the readily releasable pool, probably by clearing fused vesicle membrane at release sites. Our findings provide a unifying mechanism for the initiation of various forms of endocytosis that are critical in maintaining exocytosis.

Original languageEnglish (US)
Pages (from-to)1003-1010
Number of pages8
JournalNature Neuroscience
Volume12
Issue number8
DOIs
StatePublished - Aug 1 2009

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Calmodulin
Endocytosis
Calcium
Clathrin
Membranes
Exocytosis
Synaptic Transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wu, X. S., McNeil, B. D., Xu, J., Fan, J., Xue, L., Melicoff, E., ... Wu, L. G. (2009). Ca2+ and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal. Nature Neuroscience, 12(8), 1003-1010. https://doi.org/10.1038/nn.2355

Ca2+ and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal. / Wu, Xin Sheng; McNeil, Benjamin D.; Xu, Jianhua; Fan, Junmei; Xue, Lei; Melicoff, Ernestina; Adachi, Roberto; Bai, Li; Wu, Ling Gang.

In: Nature Neuroscience, Vol. 12, No. 8, 01.08.2009, p. 1003-1010.

Research output: Contribution to journalArticle

Wu, XS, McNeil, BD, Xu, J, Fan, J, Xue, L, Melicoff, E, Adachi, R, Bai, L & Wu, LG 2009, 'Ca2+ and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal', Nature Neuroscience, vol. 12, no. 8, pp. 1003-1010. https://doi.org/10.1038/nn.2355
Wu, Xin Sheng ; McNeil, Benjamin D. ; Xu, Jianhua ; Fan, Junmei ; Xue, Lei ; Melicoff, Ernestina ; Adachi, Roberto ; Bai, Li ; Wu, Ling Gang. / Ca2+ and calmodulin initiate all forms of endocytosis during depolarization at a nerve terminal. In: Nature Neuroscience. 2009 ; Vol. 12, No. 8. pp. 1003-1010.
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