Catechin prevents tamoxifen-induced oxidative stress and biochemical perturbations in mice

Suhel Parvez, Heena Tabassum, Hasibur Rehman, Basu Dev Banerjee, Mohammad Athar, Sheikh Raisuddin

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Natural antioxidants like catechin are now known to have a modulatory role on physiological functions and biotransformation reactions involved in the detoxification process, thereby affording protection from toxic metabolic actions of xenobiotics. Reactive oxygen intermediates have been demonstrated to play an etiological role in anticancer drug-induced toxicity. This study was performed to explore the modulatory and protective effect of catechin on the toxicity of an anticancer drug, tamoxifen (TAM) with special reference to protection against disruption of glutathione metabolizing and antioxidant enzymes. TAM treatment resulted in a significant increase in the lipid peroxidation (LPO), H2O2 generation and protein carbonyl (PC) contents in the liver and kidney as compared to controls while catechin + TAM-treated group showed significant decrease in LPO levels, H2O2 generation and PC contents in liver and kidney when compared with TAM-treated group. Non-enzymatic antioxidants like reduced glutathione (GSH) and low molecular antioxidants like ascorbic acid (AsA) also showed normalcy due to exogenous catechin administration. Catechin pre-treatment showed restoration in the level of cytochrome P450 (CYP) content and in the activities of glutathione metabolizing enzymes, viz., glutathione-S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) and other antioxidant enzymes such as, glucose-6-phosphate dehydrogenase (G6-PD), catalase (CAT) and superoxide dismutase (SOD) in both liver and kidney when compared to TAM-treated animals. The results of the study show that catechin supplementation might be helpful in abrogation of TAM toxicity during chemotherapy. Additionally, it makes it a prophylactic and preventive agent of anticancer drug-induced oxidative stress.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
Issue number2-3
StatePublished - Aug 15 2006
Externally publishedYes


  • Antioxidant enzymes
  • Catechin
  • Lipid peroxidation
  • Oxidative stress
  • Protein carbonyl
  • Tamoxifen

ASJC Scopus subject areas

  • Toxicology


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