Caveolin-1 expression is maintained in rat and human astroglioma cell lines

Patricia L Cameron, Changdan Liu, Deedee K. Smart, Stephen T. Hantus, James R. Fick, Richard S Cameron

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Caveolin-1 is the principal structural and functional component of caveolae, a plasmalemmal compartment that has been proposed to sequester lipid and protein components that participate in transmembrane signal transduction processes. Multiple studies reveal a reduction in the expression level of caveolin-1 mRNA and protein in many carcinomas as well as transformed cells. The human caveolin-1 gene is localized to a suspected tumor suppressor locus (7q31.1). Collectively, these data have been taken to imply that caveolin-1 may function in a tumor suppressor capacity. To determine if a reduction in the expression level of caveolin-1 mRNA and protein accompanied the transformation of astrocytes, we undertook studies of two transformed rat astroglial cell lines, C6 and DI TNC1, as well as several cell lines derived from human glioblastoma tumors: T98G, U87MG, U118MG, U138MG, and U373MG. Ultrastructural, immunolocalization, immunoblot, and Northern blot analyses demonstrated that caveolin-1 message and protein were expressed in all rat and human glioma cells. The localization pattern, buoyant density, and detergent-insolubility property of caveolin-1 protein were indistinguishable from that determined for nontransformed type 1 astrocytes in culture. Nucleotide sequence analyses of caveolin-1 cDNAs indicate that mutations are not present in the caveolin-1 sequence in any of the glioma cell types. Taken together with previous analyses, these data indicate that, at least for astrocytes, the process of transformation in and of itself is not solely sufficient to reduce the level of caveolin-1 expression, and that caveolin-1 expression in and of itself is not solely sufficient to prevent the acquisition of a transformed phenotype.

Original languageEnglish (US)
Pages (from-to)275-290
Number of pages16
JournalGLIA
Volume37
Issue number3
DOIs
StatePublished - Mar 1 2002

Fingerprint

Caveolin 1
Astrocytoma
Cell Line
Caveolins
Astrocytes
Glioma
Caveolae
Neoplasms
Messenger RNA
Glioblastoma
Northern Blotting
Detergents
Sequence Analysis
Signal Transduction
Complementary DNA

Keywords

  • Astroglial cells
  • Astrogliomas
  • Caveolae
  • Nervous system proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Caveolin-1 expression is maintained in rat and human astroglioma cell lines. / Cameron, Patricia L; Liu, Changdan; Smart, Deedee K.; Hantus, Stephen T.; Fick, James R.; Cameron, Richard S.

In: GLIA, Vol. 37, No. 3, 01.03.2002, p. 275-290.

Research output: Contribution to journalArticle

Cameron, Patricia L ; Liu, Changdan ; Smart, Deedee K. ; Hantus, Stephen T. ; Fick, James R. ; Cameron, Richard S. / Caveolin-1 expression is maintained in rat and human astroglioma cell lines. In: GLIA. 2002 ; Vol. 37, No. 3. pp. 275-290.
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