CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

Carla Renata Sipert, Ana Carolina de Faria Morandini, Karin Cristina da Silva Modena, Thiago Jose Dionisio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha de Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Objective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 - 10 pg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. Conclusion: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalJournal of Applied Oral Science
Volume21
Issue number2
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • CCL3 chemokine
  • Chemokines
  • CXCL12 chemokine
  • Dental pulp
  • Dental pulp inflammation
  • Fibroblasts

ASJC Scopus subject areas

  • Dentistry(all)

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