CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody

M. Wiranowska, Amyn Mohammed Rojiani, P. E. Gottschall, L. C. Moscinski, J. Johnson, S. Saporta

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have previously reported that invasiveness of mouse glioma G-26, which expresses CD44 adhesion molecule, was inhibited in vitro following treatment with anti-CD44 antibody or mouse interferon α/β (MuIFNα/β). Here, we evaluated whether the expression of transmembrane CD44 adhesion molecule and/or secretion of extracellular matrix metalloproteinases (MMPs) were affected when glioma cell invasion was inhibited. Flow cytometric evaluation of CD44 adhesion molecule expression in G-26 glioma using anti-CD44 antibody, confirmed that G-26 cells were CD44+. Following 3-day treatment with MuIFN α/β at 8×102 or 8×103 IU/ml of glioma cells, the expression of CD44 was not significantly affected as reflected by CD44+ cell number and fluorescence intensity. The pretreatment of glioma cells for 1 day with anti-CD44 antibody resulted in a 30-60% decrease of CD44 expression. This coincided with significantly (p<0.05) lower cell activity as judged by MTT assay for mitochondrial activity. The zymographic evaluation of MMP activity in the G-26 glioma cell culture showed a high level of the active form of MMP-2. This level of MMP-2 was decreased following 3 day treatment of G-26 glioma cells with either 8×103 or 8×103 IU/ml of MuIFNα/β but only the latter concentration produced statistically significant 55% decrease. However, following a 1 day treatment of G-26 glioma cells with anti-CD44 antibody, the level of active MMP-2 form was not significantly affected. These findings indicate that while the inhibitory effect of IFN on glioma invasion was accompanied by a decreased level of the active form of MMP-2 released extracellularly, the expression of the transmembrane CD44 adhesion molecule was not affected. Conversely, anti-CD44 antibody pretreatment of G-26 glioma, which led to the inhibition of glioma invasion, resulted in decreased CD44 expression and lower cell activity but had no effect on the MPP-2.

Original languageEnglish (US)
Pages (from-to)4301-4306
Number of pages6
JournalAnticancer Research
Volume20
Issue number6 B
StatePublished - Dec 1 2000
Externally publishedYes

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Keywords

  • Anti-CD44 antibody
  • CD44 adhesion molecule
  • Gelatinases
  • Glioma
  • Interferon
  • Invasiveness
  • Metalloproteinases

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Wiranowska, M., Rojiani, A. M., Gottschall, P. E., Moscinski, L. C., Johnson, J., & Saporta, S. (2000). CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody. Anticancer Research, 20(6 B), 4301-4306.