CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody

M. Wiranowska, Amyn Mohammed Rojiani, P. E. Gottschall, L. C. Moscinski, J. Johnson, S. Saporta

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have previously reported that invasiveness of mouse glioma G-26, which expresses CD44 adhesion molecule, was inhibited in vitro following treatment with anti-CD44 antibody or mouse interferon α/β (MuIFNα/β). Here, we evaluated whether the expression of transmembrane CD44 adhesion molecule and/or secretion of extracellular matrix metalloproteinases (MMPs) were affected when glioma cell invasion was inhibited. Flow cytometric evaluation of CD44 adhesion molecule expression in G-26 glioma using anti-CD44 antibody, confirmed that G-26 cells were CD44+. Following 3-day treatment with MuIFN α/β at 8×102 or 8×103 IU/ml of glioma cells, the expression of CD44 was not significantly affected as reflected by CD44+ cell number and fluorescence intensity. The pretreatment of glioma cells for 1 day with anti-CD44 antibody resulted in a 30-60% decrease of CD44 expression. This coincided with significantly (p<0.05) lower cell activity as judged by MTT assay for mitochondrial activity. The zymographic evaluation of MMP activity in the G-26 glioma cell culture showed a high level of the active form of MMP-2. This level of MMP-2 was decreased following 3 day treatment of G-26 glioma cells with either 8×103 or 8×103 IU/ml of MuIFNα/β but only the latter concentration produced statistically significant 55% decrease. However, following a 1 day treatment of G-26 glioma cells with anti-CD44 antibody, the level of active MMP-2 form was not significantly affected. These findings indicate that while the inhibitory effect of IFN on glioma invasion was accompanied by a decreased level of the active form of MMP-2 released extracellularly, the expression of the transmembrane CD44 adhesion molecule was not affected. Conversely, anti-CD44 antibody pretreatment of G-26 glioma, which led to the inhibition of glioma invasion, resulted in decreased CD44 expression and lower cell activity but had no effect on the MPP-2.

Original languageEnglish (US)
Pages (from-to)4301-4306
Number of pages6
JournalAnticancer Research
Volume20
Issue number6 B
StatePublished - Dec 1 2000
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 2
Glioma
Interferons
Anti-Idiotypic Antibodies
Matrix Metalloproteinases
Gastrin-Secreting Cells
Extracellular Matrix
Cell Culture Techniques
Cell Count
Fluorescence

Keywords

  • Anti-CD44 antibody
  • CD44 adhesion molecule
  • Gelatinases
  • Glioma
  • Interferon
  • Invasiveness
  • Metalloproteinases

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Wiranowska, M., Rojiani, A. M., Gottschall, P. E., Moscinski, L. C., Johnson, J., & Saporta, S. (2000). CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody. Anticancer Research, 20(6 B), 4301-4306.

CD44 expression and MMP-2 secretion by mouse glioma cells : Effect of interferon and anti-CD44 antibody. / Wiranowska, M.; Rojiani, Amyn Mohammed; Gottschall, P. E.; Moscinski, L. C.; Johnson, J.; Saporta, S.

In: Anticancer Research, Vol. 20, No. 6 B, 01.12.2000, p. 4301-4306.

Research output: Contribution to journalArticle

Wiranowska, M, Rojiani, AM, Gottschall, PE, Moscinski, LC, Johnson, J & Saporta, S 2000, 'CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody', Anticancer Research, vol. 20, no. 6 B, pp. 4301-4306.
Wiranowska M, Rojiani AM, Gottschall PE, Moscinski LC, Johnson J, Saporta S. CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody. Anticancer Research. 2000 Dec 1;20(6 B):4301-4306.
Wiranowska, M. ; Rojiani, Amyn Mohammed ; Gottschall, P. E. ; Moscinski, L. C. ; Johnson, J. ; Saporta, S. / CD44 expression and MMP-2 secretion by mouse glioma cells : Effect of interferon and anti-CD44 antibody. In: Anticancer Research. 2000 ; Vol. 20, No. 6 B. pp. 4301-4306.
@article{0b7589d6f0e0472a84676f14b18619c2,
title = "CD44 expression and MMP-2 secretion by mouse glioma cells: Effect of interferon and anti-CD44 antibody",
abstract = "We have previously reported that invasiveness of mouse glioma G-26, which expresses CD44 adhesion molecule, was inhibited in vitro following treatment with anti-CD44 antibody or mouse interferon α/β (MuIFNα/β). Here, we evaluated whether the expression of transmembrane CD44 adhesion molecule and/or secretion of extracellular matrix metalloproteinases (MMPs) were affected when glioma cell invasion was inhibited. Flow cytometric evaluation of CD44 adhesion molecule expression in G-26 glioma using anti-CD44 antibody, confirmed that G-26 cells were CD44+. Following 3-day treatment with MuIFN α/β at 8×102 or 8×103 IU/ml of glioma cells, the expression of CD44 was not significantly affected as reflected by CD44+ cell number and fluorescence intensity. The pretreatment of glioma cells for 1 day with anti-CD44 antibody resulted in a 30-60{\%} decrease of CD44 expression. This coincided with significantly (p<0.05) lower cell activity as judged by MTT assay for mitochondrial activity. The zymographic evaluation of MMP activity in the G-26 glioma cell culture showed a high level of the active form of MMP-2. This level of MMP-2 was decreased following 3 day treatment of G-26 glioma cells with either 8×103 or 8×103 IU/ml of MuIFNα/β but only the latter concentration produced statistically significant 55{\%} decrease. However, following a 1 day treatment of G-26 glioma cells with anti-CD44 antibody, the level of active MMP-2 form was not significantly affected. These findings indicate that while the inhibitory effect of IFN on glioma invasion was accompanied by a decreased level of the active form of MMP-2 released extracellularly, the expression of the transmembrane CD44 adhesion molecule was not affected. Conversely, anti-CD44 antibody pretreatment of G-26 glioma, which led to the inhibition of glioma invasion, resulted in decreased CD44 expression and lower cell activity but had no effect on the MPP-2.",
keywords = "Anti-CD44 antibody, CD44 adhesion molecule, Gelatinases, Glioma, Interferon, Invasiveness, Metalloproteinases",
author = "M. Wiranowska and Rojiani, {Amyn Mohammed} and Gottschall, {P. E.} and Moscinski, {L. C.} and J. Johnson and S. Saporta",
year = "2000",
month = "12",
day = "1",
language = "English (US)",
volume = "20",
pages = "4301--4306",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "6 B",

}

TY - JOUR

T1 - CD44 expression and MMP-2 secretion by mouse glioma cells

T2 - Effect of interferon and anti-CD44 antibody

AU - Wiranowska, M.

AU - Rojiani, Amyn Mohammed

AU - Gottschall, P. E.

AU - Moscinski, L. C.

AU - Johnson, J.

AU - Saporta, S.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - We have previously reported that invasiveness of mouse glioma G-26, which expresses CD44 adhesion molecule, was inhibited in vitro following treatment with anti-CD44 antibody or mouse interferon α/β (MuIFNα/β). Here, we evaluated whether the expression of transmembrane CD44 adhesion molecule and/or secretion of extracellular matrix metalloproteinases (MMPs) were affected when glioma cell invasion was inhibited. Flow cytometric evaluation of CD44 adhesion molecule expression in G-26 glioma using anti-CD44 antibody, confirmed that G-26 cells were CD44+. Following 3-day treatment with MuIFN α/β at 8×102 or 8×103 IU/ml of glioma cells, the expression of CD44 was not significantly affected as reflected by CD44+ cell number and fluorescence intensity. The pretreatment of glioma cells for 1 day with anti-CD44 antibody resulted in a 30-60% decrease of CD44 expression. This coincided with significantly (p<0.05) lower cell activity as judged by MTT assay for mitochondrial activity. The zymographic evaluation of MMP activity in the G-26 glioma cell culture showed a high level of the active form of MMP-2. This level of MMP-2 was decreased following 3 day treatment of G-26 glioma cells with either 8×103 or 8×103 IU/ml of MuIFNα/β but only the latter concentration produced statistically significant 55% decrease. However, following a 1 day treatment of G-26 glioma cells with anti-CD44 antibody, the level of active MMP-2 form was not significantly affected. These findings indicate that while the inhibitory effect of IFN on glioma invasion was accompanied by a decreased level of the active form of MMP-2 released extracellularly, the expression of the transmembrane CD44 adhesion molecule was not affected. Conversely, anti-CD44 antibody pretreatment of G-26 glioma, which led to the inhibition of glioma invasion, resulted in decreased CD44 expression and lower cell activity but had no effect on the MPP-2.

AB - We have previously reported that invasiveness of mouse glioma G-26, which expresses CD44 adhesion molecule, was inhibited in vitro following treatment with anti-CD44 antibody or mouse interferon α/β (MuIFNα/β). Here, we evaluated whether the expression of transmembrane CD44 adhesion molecule and/or secretion of extracellular matrix metalloproteinases (MMPs) were affected when glioma cell invasion was inhibited. Flow cytometric evaluation of CD44 adhesion molecule expression in G-26 glioma using anti-CD44 antibody, confirmed that G-26 cells were CD44+. Following 3-day treatment with MuIFN α/β at 8×102 or 8×103 IU/ml of glioma cells, the expression of CD44 was not significantly affected as reflected by CD44+ cell number and fluorescence intensity. The pretreatment of glioma cells for 1 day with anti-CD44 antibody resulted in a 30-60% decrease of CD44 expression. This coincided with significantly (p<0.05) lower cell activity as judged by MTT assay for mitochondrial activity. The zymographic evaluation of MMP activity in the G-26 glioma cell culture showed a high level of the active form of MMP-2. This level of MMP-2 was decreased following 3 day treatment of G-26 glioma cells with either 8×103 or 8×103 IU/ml of MuIFNα/β but only the latter concentration produced statistically significant 55% decrease. However, following a 1 day treatment of G-26 glioma cells with anti-CD44 antibody, the level of active MMP-2 form was not significantly affected. These findings indicate that while the inhibitory effect of IFN on glioma invasion was accompanied by a decreased level of the active form of MMP-2 released extracellularly, the expression of the transmembrane CD44 adhesion molecule was not affected. Conversely, anti-CD44 antibody pretreatment of G-26 glioma, which led to the inhibition of glioma invasion, resulted in decreased CD44 expression and lower cell activity but had no effect on the MPP-2.

KW - Anti-CD44 antibody

KW - CD44 adhesion molecule

KW - Gelatinases

KW - Glioma

KW - Interferon

KW - Invasiveness

KW - Metalloproteinases

UR - http://www.scopus.com/inward/record.url?scp=0034477147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034477147&partnerID=8YFLogxK

M3 - Article

C2 - 11205262

AN - SCOPUS:0034477147

VL - 20

SP - 4301

EP - 4306

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 6 B

ER -