CD8+ T-cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes

Diana L. Martin, D. Brent Weatherly, Susana A. Laucella, Melissa A. Cabinian, Matthew Tyler Crim, Susan Sullivan, Mark Heiges, Sarah H. Craven, Charles S. Rosenberg, Matthew H. Collins, Alessandro Sette, Miriam Postan, Rick L. Tarleton

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

CD8+ T cells are crucial for control of a number of medically important protozoan parasites, including Trypanosoma cruzi, the agent of human Chagas disease. Yet, in contrast to the wealth of information from viral and bacterial infections, little is known about the antigen specificity or the general development of effector and memory T-cell responses in hosts infected with protozoans. In this study we report on a wide-scale screen for the dominant parasite peptides recognized by CD8+ T cells in T. cruzi-infected mice and humans. This analysis demonstrates that in both hosts the CD8 + T-cell response is highly focused on epitopes encoded by members of the large trans-sialidase family of genes. Responses to a restricted set of immunodominant peptides were especially pronounced in T. cruzi-infected mice, with more than 30% of the CD8+ T-cell response at the peak of infection specific for two major groups of transsialidase peptides. Experimental models also demonstrated that the dominance patterns vary depending on the infective strain of T. cruzi, suggesting that immune evasion may be occurring at a population rather than single-parasite level.

Original languageEnglish (US)
Pages (from-to)731-740
Number of pages10
JournalPLoS Pathogens
Volume2
Issue number8
DOIs
StatePublished - Sep 4 2006
Externally publishedYes

Fingerprint

Trypanosoma cruzi
Epitopes
T-Lymphocytes
Parasites
Peptides
Immune Evasion
Chagas Disease
Virus Diseases
Bacterial Infections
Theoretical Models
trans-sialidase
Antigens
Infection
Population
Genes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Martin, D. L., Weatherly, D. B., Laucella, S. A., Cabinian, M. A., Crim, M. T., Sullivan, S., ... Tarleton, R. L. (2006). CD8+ T-cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes. PLoS Pathogens, 2(8), 731-740. https://doi.org/10.1371/journal.ppat.0020077

CD8+ T-cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes. / Martin, Diana L.; Weatherly, D. Brent; Laucella, Susana A.; Cabinian, Melissa A.; Crim, Matthew Tyler; Sullivan, Susan; Heiges, Mark; Craven, Sarah H.; Rosenberg, Charles S.; Collins, Matthew H.; Sette, Alessandro; Postan, Miriam; Tarleton, Rick L.

In: PLoS Pathogens, Vol. 2, No. 8, 04.09.2006, p. 731-740.

Research output: Contribution to journalArticle

Martin, DL, Weatherly, DB, Laucella, SA, Cabinian, MA, Crim, MT, Sullivan, S, Heiges, M, Craven, SH, Rosenberg, CS, Collins, MH, Sette, A, Postan, M & Tarleton, RL 2006, 'CD8+ T-cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes', PLoS Pathogens, vol. 2, no. 8, pp. 731-740. https://doi.org/10.1371/journal.ppat.0020077
Martin, Diana L. ; Weatherly, D. Brent ; Laucella, Susana A. ; Cabinian, Melissa A. ; Crim, Matthew Tyler ; Sullivan, Susan ; Heiges, Mark ; Craven, Sarah H. ; Rosenberg, Charles S. ; Collins, Matthew H. ; Sette, Alessandro ; Postan, Miriam ; Tarleton, Rick L. / CD8+ T-cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes. In: PLoS Pathogens. 2006 ; Vol. 2, No. 8. pp. 731-740.
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