cDNA cloning and expression of oxysterol-binding protein, an oligomer with a potential leucine zipper

P. A. Dawson, N. D. Ridgway, Clive A. Slaughter, M. S. Brown, J. L. Goldstein

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Feedback repression of the genes encoding the low density lipoprotein receptor and several enzymes of the cholesterol biosynthetic pathway is mediated by 25-hydroxycholesterol and other oxysterols. In this study, we have cloned a rabbit cDNA encoding an oxysterol-binding protein that may play a role in this regulation. The predicted amino acid sequence revealed a protein of 809 amino acids with two distinctive features: 1) a glycine- and alanine-rich region (63% of 80 residues) at the NH2 terminus, and 2) a 35-residue leucine zipper motif that may mediate the previously observed oligomerization of the protein. When transfected into simian COS cells, the rabbit cDNA produced a protein that exhibited the same affinity and specificity for sterols as the previously purified hamster liver protein. Immunoblotting analysis showed that the rabbit cDNA encodes both the 96- and 101-kilodalton forms of the oxysterol-binding protein that were previously observed. The availability of an expressible cDNA for the oxysterol-binding protein should help elucidate its role in sterol metabolism.

Original languageEnglish (US)
Pages (from-to)16798-16803
Number of pages6
JournalJournal of Biological Chemistry
Volume264
Issue number28
StatePublished - Jan 1 1989

Fingerprint

Leucine Zippers
Cloning
Oligomers
Organism Cloning
Complementary DNA
Sterols
Rabbits
Proteins
Amino Acids
Oligomerization
Gene encoding
LDL Receptors
COS Cells
Biosynthetic Pathways
Immunoblotting
Metabolism
Cricetinae
Alanine
Liver
Glycine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

cDNA cloning and expression of oxysterol-binding protein, an oligomer with a potential leucine zipper. / Dawson, P. A.; Ridgway, N. D.; Slaughter, Clive A.; Brown, M. S.; Goldstein, J. L.

In: Journal of Biological Chemistry, Vol. 264, No. 28, 01.01.1989, p. 16798-16803.

Research output: Contribution to journalArticle

Dawson, P. A. ; Ridgway, N. D. ; Slaughter, Clive A. ; Brown, M. S. ; Goldstein, J. L. / cDNA cloning and expression of oxysterol-binding protein, an oligomer with a potential leucine zipper. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 28. pp. 16798-16803.
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