cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome

Akihiko Saito, Takeshi K. Watanabe, Yoshikazu Shimada, Tsutomu Fujiwara, Clive A. Slaughter, George N. Demartino, Nobuyuki Tanahashi, Keiji Tanaka

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We have employed cDNA cloning to deduce the complete primary structures of p44.5 and p55, two subunits of PA700, a 700-kDa multisubunit regulatory complex of the human 26S proteasome. These polypeptides consist of 422 and 456 amino acids with calculated molecular masses of 47,463 and 52,903, and isoelectric points of 6.06 and 7.56, respectively. Computer-assisted homology analysis revealed high sequence similarities of p44.5 and p55 with yeast proteins whose functions are yet unknown. Disruption of the yeast genes, termed NAS4 and NAS5 (non-ATPase subunits 4 and 5), resulted in lethality, indicating that each of the two subunits is essential for proliferation of yeast cells.

Original languageEnglish (US)
Pages (from-to)241-250
Number of pages10
JournalGene
Volume203
Issue number2
DOIs
StatePublished - Dec 12 1997
Externally publishedYes

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Keywords

  • Gene disruption
  • Non-ATPase subunit
  • PA700
  • Ubiquitin
  • Yeast

ASJC Scopus subject areas

  • Genetics

Cite this

Saito, A., Watanabe, T. K., Shimada, Y., Fujiwara, T., Slaughter, C. A., Demartino, G. N., Tanahashi, N., & Tanaka, K. (1997). cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome. Gene, 203(2), 241-250. https://doi.org/10.1016/S0378-1119(97)00524-6