cDNA cloning and functional analysis of the p97 subunit of the 265 proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11

Chizuko Tsurumi, Yoshihisa Shimizu, Mihoro Saeki, Seishi Kato, George N. Demartino, Clive A. Slaughter, Masahiro Fujimuro, Hideyoshi Yokosawa, Moto O. Yamasaki, Klavs B. Hendil, Akio Toh-E, Nobuyuki Tanahashi, Keiji Tanaka

Research output: Contribution to journalArticle

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Abstract

Molecular cloning of cDNA for a new regulatory subunit, designated p97, of the human 26S proteasome showed that the polypeptide consists of 908 amino acid residues with a calculated molecular mass of 100184 Da and an isoelectric point of 4.94. Computer analysis showed that p97 is very similar to type-1 tumor-necrosis-factor(TNF)-receptor-associated protein (TRAP)-2 and 55.11, both of which were identified recently as binding proteins of the cytoplasmic domain of type-1 TNF receptor by yeast two-hybrid screening. This finding suggests that the 26S proteasome might serve as a mediator molecule in the TNF signaling pathway in cells. Computer-assisted similarity analysis also revealed the high sequence similarity of p97 with a yeast protein whose function is yet unknown, the gene for which is here termed NASI (non-ATPase subunit 1). Disruption of NAS1 resulted in several phenotypes, including lethality and temperature-sensitive growth, depending on the genetic background of the cells used. The human p97 cDNA suppressed the growth defect of nas1 disruptant cells, when expressed from single-copy or multicopy vectors, indicating that p97 is functionally equivalent to yeast Nas1p. Culturing of the temperature sensitive nas1 cells at the restrictive temperature promoted the accumulation polyubiquitinated cellular proteins, implying that the 26S proteasome requires a functional Nas1p subunit for ubiquitin-dependent proteolysis. These results indicate that p97/Nas1p plays an important regulatory role in the function of the 26S proteasome.

Original languageEnglish (US)
Pages (from-to)912-921
Number of pages10
JournalEuropean Journal of Biochemistry
Volume239
Issue number3
DOIs
StatePublished - Jan 1 1996

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Receptors, Tumor Necrosis Factor, Type I
Functional analysis
Cloning
Proteasome Endopeptidase Complex
Organism Cloning
Complementary DNA
Peptides
Yeast
Temperature
Proteins
Yeasts
Proteolysis
Fungal Proteins
Isoelectric Point
Molecular mass
Molecular Cloning
Growth
Ubiquitin
Carrier Proteins
Screening

Keywords

  • Proteasome
  • Type-1 tumor-necrosis-factor receptor
  • Ubiquitin
  • p97 subunit

ASJC Scopus subject areas

  • Biochemistry

Cite this

cDNA cloning and functional analysis of the p97 subunit of the 265 proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11. / Tsurumi, Chizuko; Shimizu, Yoshihisa; Saeki, Mihoro; Kato, Seishi; Demartino, George N.; Slaughter, Clive A.; Fujimuro, Masahiro; Yokosawa, Hideyoshi; Yamasaki, Moto O.; Hendil, Klavs B.; Toh-E, Akio; Tanahashi, Nobuyuki; Tanaka, Keiji.

In: European Journal of Biochemistry, Vol. 239, No. 3, 01.01.1996, p. 912-921.

Research output: Contribution to journalArticle

Tsurumi, C, Shimizu, Y, Saeki, M, Kato, S, Demartino, GN, Slaughter, CA, Fujimuro, M, Yokosawa, H, Yamasaki, MO, Hendil, KB, Toh-E, A, Tanahashi, N & Tanaka, K 1996, 'cDNA cloning and functional analysis of the p97 subunit of the 265 proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11', European Journal of Biochemistry, vol. 239, no. 3, pp. 912-921. https://doi.org/10.1111/j.1432-1033.1996.0912u.x
Tsurumi, Chizuko ; Shimizu, Yoshihisa ; Saeki, Mihoro ; Kato, Seishi ; Demartino, George N. ; Slaughter, Clive A. ; Fujimuro, Masahiro ; Yokosawa, Hideyoshi ; Yamasaki, Moto O. ; Hendil, Klavs B. ; Toh-E, Akio ; Tanahashi, Nobuyuki ; Tanaka, Keiji. / cDNA cloning and functional analysis of the p97 subunit of the 265 proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11. In: European Journal of Biochemistry. 1996 ; Vol. 239, No. 3. pp. 912-921.
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abstract = "Molecular cloning of cDNA for a new regulatory subunit, designated p97, of the human 26S proteasome showed that the polypeptide consists of 908 amino acid residues with a calculated molecular mass of 100184 Da and an isoelectric point of 4.94. Computer analysis showed that p97 is very similar to type-1 tumor-necrosis-factor(TNF)-receptor-associated protein (TRAP)-2 and 55.11, both of which were identified recently as binding proteins of the cytoplasmic domain of type-1 TNF receptor by yeast two-hybrid screening. This finding suggests that the 26S proteasome might serve as a mediator molecule in the TNF signaling pathway in cells. Computer-assisted similarity analysis also revealed the high sequence similarity of p97 with a yeast protein whose function is yet unknown, the gene for which is here termed NASI (non-ATPase subunit 1). Disruption of NAS1 resulted in several phenotypes, including lethality and temperature-sensitive growth, depending on the genetic background of the cells used. The human p97 cDNA suppressed the growth defect of nas1 disruptant cells, when expressed from single-copy or multicopy vectors, indicating that p97 is functionally equivalent to yeast Nas1p. Culturing of the temperature sensitive nas1 cells at the restrictive temperature promoted the accumulation polyubiquitinated cellular proteins, implying that the 26S proteasome requires a functional Nas1p subunit for ubiquitin-dependent proteolysis. These results indicate that p97/Nas1p plays an important regulatory role in the function of the 26S proteasome.",
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AU - Tsurumi, Chizuko

AU - Shimizu, Yoshihisa

AU - Saeki, Mihoro

AU - Kato, Seishi

AU - Demartino, George N.

AU - Slaughter, Clive A.

AU - Fujimuro, Masahiro

AU - Yokosawa, Hideyoshi

AU - Yamasaki, Moto O.

AU - Hendil, Klavs B.

AU - Toh-E, Akio

AU - Tanahashi, Nobuyuki

AU - Tanaka, Keiji

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