cDNA cloning of p40, a regulatory subunit of the human 26S proteasome, and a homolog of the Mov-34 gene product

Chizuko Tsurumi, George N. Demartino, Clive A. Slaughter, Naoki Shimbara, Keiji Tanaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The nucleotide sequence of a cDNA that encodes a new regulatory subunit, named p40, of the 26S proteasome of human hepatoblastoma HepG2 cells has been determined. The polypeptide predicted from the open reading frame consists of 324 amino acid residues with a calculated molecular mass of 37020 and isoelectric point of 6.03. A KEKE motif, consisting of a very hydrophilic domain rich in 'alternating' lysine (positive) and glutamate (negative) residues, is present in the C-terminus of p40. The overall structure of p40 is homologous to that of the mouse Mov-34 gene product, whose gene disruption by proviral integration results in a recessive embryonic lethality. Thus the p40/Mov-34 protein is a novel essential regulatory subunit of the human 26S proteasome.

Original languageEnglish (US)
Pages (from-to)600-608
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number2
DOIs
StatePublished - May 16 1995
Externally publishedYes

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Cloning
Organism Cloning
Complementary DNA
Genes
Hepatoblastoma
Isoelectric Point
Hep G2 Cells
Molecular mass
Open Reading Frames
Lysine
Glutamic Acid
Nucleotides
Amino Acids
Peptides
adjuvant P40
ATP dependent 26S protease
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

cDNA cloning of p40, a regulatory subunit of the human 26S proteasome, and a homolog of the Mov-34 gene product. / Tsurumi, Chizuko; Demartino, George N.; Slaughter, Clive A.; Shimbara, Naoki; Tanaka, Keiji.

In: Biochemical and Biophysical Research Communications, Vol. 210, No. 2, 16.05.1995, p. 600-608.

Research output: Contribution to journalArticle

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