CDNA cloning of p42, a shared subunit of two proteasome regulatory proteins, reveals a novel member of the AAA protein family

Tsutomua Fujiwara, Takeshi K. Watanabe, Keiji Tanaka, Clive A. Slaughter, George N. DeMartino

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have employed cDNA cloning to deduce the complete primary structure of p42, a protein previously identified as a common subunit of two proteasome regulatory proteins: PA700, a 700,000-Da multisubunit complex that binds to the proteasome and promotes the ATP-dependent degradation of ubiquitinated proteins, and modulator, a 250,000-Da PA700-dependent proteasome activator. Computer analysis reveals that p42 is a novel member of a large protein family characterized by a conserved 200 amino acid domain which contains a consensus sequence for ATP binding. Five other members of this family, termed AAA proteins (ATPases associated with a variety of cellular activities) are also subunits of PA700. Gel filtration chromatography was employed to determine the qualitative and quantitative zdistribution of p42 in crude soluble lysates of bovine red blood cells. These studies demonstrated that p42 was found in two multi-protein complexes: the 26S proteasome (formed from the 20S proteasome and PA700) and the modulator. These results establish the identity of a new protein involved in the regulation of proteasome function and indicate that this protein is found in at least two different protein complexes.

Original languageEnglish (US)
Pages (from-to)184-188
Number of pages5
JournalFEBS Letters
Volume387
Issue number2-3
DOIs
StatePublished - Jun 3 1996

Fingerprint

Cloning
Proteasome Endopeptidase Complex
Organism Cloning
Proteins
Modulators
Adenosine Triphosphate
Ubiquitinated Proteins
Consensus Sequence
Chromatography
Gel Chromatography
Adenosine Triphosphatases
Blood
Complementary DNA
Erythrocytes
Gels
Cells
Amino Acids
Degradation

Keywords

  • AAA protein family
  • ATPase
  • Modulator
  • PA700
  • Proteasome
  • Sug2p
  • p42

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

CDNA cloning of p42, a shared subunit of two proteasome regulatory proteins, reveals a novel member of the AAA protein family. / Fujiwara, Tsutomua; Watanabe, Takeshi K.; Tanaka, Keiji; Slaughter, Clive A.; DeMartino, George N.

In: FEBS Letters, Vol. 387, No. 2-3, 03.06.1996, p. 184-188.

Research output: Contribution to journalArticle

Fujiwara, Tsutomua ; Watanabe, Takeshi K. ; Tanaka, Keiji ; Slaughter, Clive A. ; DeMartino, George N. / CDNA cloning of p42, a shared subunit of two proteasome regulatory proteins, reveals a novel member of the AAA protein family. In: FEBS Letters. 1996 ; Vol. 387, No. 2-3. pp. 184-188.
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