cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines

C. Betsholtz, A. Johnsson, C. H. Heldin, B. Westermark, P. Lind, M. S. Urdea, R. Eddy, T. B. Shows, K. Philpott, A. L. Mellor

Research output: Contribution to journalArticle

518 Citations (Scopus)

Abstract

The amino-acid sequence of the precursor of the human tumour cell line-derived platelet-derived growth factor (PDGF) A-chain has been deduced from complementary DNA clones and the gene localized to chromosome 7. The protein shows extensive homology to the PDGF B-chain precursor. Expression of the PDGF A-chain gene is independent of that of the PDGF B-chain in a number of human tumour cell lines, and secretion of a PDGF-like growth factor of relative molecular mass 31,000 correlates with expression of A- but not B-chain messenger RNA.

Original languageEnglish (US)
Pages (from-to)695-699
Number of pages5
JournalNature
Volume320
Issue number6064
DOIs
StatePublished - Dec 1 1986

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Proto-Oncogene Proteins c-sis
Tumor Cell Line
Complementary DNA
Chromosomes, Human, Pair 7
Platelet-Derived Growth Factor
Genes
Amino Acid Sequence
Intercellular Signaling Peptides and Proteins
Clone Cells
Messenger RNA
Proteins
platelet-derived growth factor A

ASJC Scopus subject areas

  • General

Cite this

Betsholtz, C., Johnsson, A., Heldin, C. H., Westermark, B., Lind, P., Urdea, M. S., ... Mellor, A. L. (1986). cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature, 320(6064), 695-699. https://doi.org/10.1038/320695a0

cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. / Betsholtz, C.; Johnsson, A.; Heldin, C. H.; Westermark, B.; Lind, P.; Urdea, M. S.; Eddy, R.; Shows, T. B.; Philpott, K.; Mellor, A. L.

In: Nature, Vol. 320, No. 6064, 01.12.1986, p. 695-699.

Research output: Contribution to journalArticle

Betsholtz, C, Johnsson, A, Heldin, CH, Westermark, B, Lind, P, Urdea, MS, Eddy, R, Shows, TB, Philpott, K & Mellor, AL 1986, 'cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines', Nature, vol. 320, no. 6064, pp. 695-699. https://doi.org/10.1038/320695a0
Betsholtz, C. ; Johnsson, A. ; Heldin, C. H. ; Westermark, B. ; Lind, P. ; Urdea, M. S. ; Eddy, R. ; Shows, T. B. ; Philpott, K. ; Mellor, A. L. / cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. In: Nature. 1986 ; Vol. 320, No. 6064. pp. 695-699.
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