cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family

Xiang Wu, Puttur D Prasad, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

288 Citations (Scopus)

Abstract

We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.

Original languageEnglish (US)
Pages (from-to)589-595
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number3
DOIs
StatePublished - May 29 1998

Fingerprint

Cations
Complementary DNA
Cells
1-Methyl-4-phenylpyridinium
Amino Acids
Cell Line
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Tetraethylammonium
Methamphetamine
Neurotoxins
Trophoblasts
Human Chromosomes
Chromosomes
HeLa Cells
Amino Acid Sequence
Exons
Genes
Tissue
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family. / Wu, Xiang; Prasad, Puttur D; Leibach, Frederick H.; Ganapathy, Vadivel.

In: Biochemical and Biophysical Research Communications, Vol. 246, No. 3, 29.05.1998, p. 589-595.

Research output: Contribution to journalArticle

@article{b3e3c61b30f7420a9b07282d2868b329,
title = "cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family",
abstract = "We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.",
author = "Xiang Wu and Prasad, {Puttur D} and Leibach, {Frederick H.} and Vadivel Ganapathy",
year = "1998",
month = "5",
day = "29",
doi = "10.1006/bbrc.1998.8669",
language = "English (US)",
volume = "246",
pages = "589--595",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family

AU - Wu, Xiang

AU - Prasad, Puttur D

AU - Leibach, Frederick H.

AU - Ganapathy, Vadivel

PY - 1998/5/29

Y1 - 1998/5/29

N2 - We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.

AB - We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.

UR - http://www.scopus.com/inward/record.url?scp=0032577202&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032577202&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.8669

DO - 10.1006/bbrc.1998.8669

M3 - Article

VL - 246

SP - 589

EP - 595

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -