cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family

Xiang Wu, Puttur D. Prasad, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

292 Scopus citations


We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.

Original languageEnglish (US)
Pages (from-to)589-595
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - May 29 1998


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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