TY - JOUR
T1 - cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family
AU - Wu, Xiang
AU - Prasad, Puttur D.
AU - Leibach, Frederick H.
AU - Ganapathy, Vadivel
N1 - Funding Information:
This work was supported by National Institutes of Health Grants DA 10045, HD 24451, and HD 33347. We thank Ida O. Walker for excellent secretarial assistance.
PY - 1998/5/29
Y1 - 1998/5/29
N2 - We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.
AB - We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3. When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine, compete for the OCTN2-mediated transport process.
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U2 - 10.1006/bbrc.1998.8669
DO - 10.1006/bbrc.1998.8669
M3 - Article
C2 - 9618255
AN - SCOPUS:0032577202
SN - 0006-291X
VL - 246
SP - 589
EP - 595
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -