TY - JOUR
T1 - CellGeo
T2 - A computational platform for the analysis of shape changes in cells with complex geometries
AU - Tsygankov, Denis
AU - Bilancia, Colleen G.
AU - Vitriol, Eric A.
AU - Hahn, Klaus M.
AU - Peifer, Mark
AU - Elston, Timothy C.
PY - 2014/2/3
Y1 - 2014/2/3
N2 - Cell biologists increasingly rely on computer-aided image analysis, allowing them to collect precise, unbiased quantitative results. However, despite great progress in image processing and computer vision, current computational approaches fail to address many key aspects of cell behavior, including the cell protrusions that guide cell migration and drive morphogenesis. We developed the open source MATLAB application CellGeo, a user-friendly computational platform to allow simultaneous, automated tracking and analysis of dynamic changes in cell shape, including protrusions ranging from filopodia to lamellipodia. Our method maps an arbitrary cell shape onto a tree graph that, unlike traditional skeletonization algorithms, preserves complex boundary features. CellGeo allows rigorous but flexible definition and accurate automated detection and tracking of geometric features of interest. We demonstrate CellGeo's utility by deriving new insights into (a) the roles of Diaphanous, Enabled, and Capping protein in regulating filopodia and lamellipodia dynamics in Drosophila melanogaster cells and (b) the dynamic properties of growth cones in catecholaminergic a- differentiated neuroblastoma cells.
AB - Cell biologists increasingly rely on computer-aided image analysis, allowing them to collect precise, unbiased quantitative results. However, despite great progress in image processing and computer vision, current computational approaches fail to address many key aspects of cell behavior, including the cell protrusions that guide cell migration and drive morphogenesis. We developed the open source MATLAB application CellGeo, a user-friendly computational platform to allow simultaneous, automated tracking and analysis of dynamic changes in cell shape, including protrusions ranging from filopodia to lamellipodia. Our method maps an arbitrary cell shape onto a tree graph that, unlike traditional skeletonization algorithms, preserves complex boundary features. CellGeo allows rigorous but flexible definition and accurate automated detection and tracking of geometric features of interest. We demonstrate CellGeo's utility by deriving new insights into (a) the roles of Diaphanous, Enabled, and Capping protein in regulating filopodia and lamellipodia dynamics in Drosophila melanogaster cells and (b) the dynamic properties of growth cones in catecholaminergic a- differentiated neuroblastoma cells.
UR - http://www.scopus.com/inward/record.url?scp=84893453773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893453773&partnerID=8YFLogxK
U2 - 10.1083/jcb.201306067
DO - 10.1083/jcb.201306067
M3 - Article
C2 - 24493591
AN - SCOPUS:84893453773
SN - 0021-9525
VL - 204
SP - 443
EP - 460
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -