Cellular distribution of insulin-degrading enzyme gene expression. Comparison with insulin and insulin-like growth factor receptors

C. A. Bondy, J. Zhou, Edward Chin, R. R. Reinhardt, L. Ding, R. A. Roth

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Insulin-degrading enzyme (IDE) hydrolyzes both insulin and IGFs and has been proposed to play a role in signal termination after binding of these peptides to their receptors. In situ hybridization was used to investigate the cellular distribution of IDE mRNA and to compare it with insulin receptor (IR) and IGF-I receptor (IGFR) gene expression in serial thin sections from a variety of tissues in embryonic and adult rats. IDE mRNA is highly abundant in kidney and liver, tissues known to play a role in insulin degradation. IDE and IR mRNAs are highly coexpressed in brown fat and liver. The highest level IDE gene expression, on a per cell basis, is found in germinal epithelium. IDE and IGFR mRNAs are colocalized in oocytes, while IDE is colocalized with the IGF-II receptor in spermatocytes, suggesting that IDE may be involved with degradation of IGF-II in the testis. In summary, IDE expression demonstrates significant anatomical correlation with insulin/IGF receptors. These data are compatible with a role for IDE in degrading insulin and IGFs after they bind to and are internalized with their respective receptors and may also suggest a novel role for IDE in germ cells.

Original languageEnglish (US)
Pages (from-to)966-973
Number of pages8
JournalJournal of Clinical Investigation
Volume93
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Insulysin
Somatomedin Receptors
Insulin
Gene Expression
Insulin Receptor
IGF Type 1 Receptor
Messenger RNA
IGF Type 2 Receptor
Insulin-Like Growth Factor II
Brown Adipose Tissue
Spermatocytes
Liver
Germ Cells
Oocytes
In Situ Hybridization
Testis

Keywords

  • in situ hybridization
  • mRNA
  • oocyte
  • proteolysis
  • spermatocyte

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cellular distribution of insulin-degrading enzyme gene expression. Comparison with insulin and insulin-like growth factor receptors. / Bondy, C. A.; Zhou, J.; Chin, Edward; Reinhardt, R. R.; Ding, L.; Roth, R. A.

In: Journal of Clinical Investigation, Vol. 93, No. 3, 01.01.1994, p. 966-973.

Research output: Contribution to journalArticle

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AU - Roth, R. A.

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