Cellular energetics in the preconditioned state

Protective role for phosphotransfer reactions captured by 18O-assisted 31P NMR

Darko Pucar, Petras P. Dzeja, Peter Bast, Nenad Juranic, Slobodan Macura, Andre Terzic

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Cell survival is critically dependent on the preservation of cellular bioenergetics. However, the metabolic mechanisms that confer resistance to injury are poorly understood. Phosphotransfer reactions integrate ATP-consuming with ATP-producing processes and could thereby contribute to the generation of a protective phenotype. Here, we used ischemic preconditioning to induce a stress-tolerant state and 18O-assisted 31P nuclear magnetic resonance spectroscopy to capture intracellular phosphotransfer dynamics. Preconditioning of isolated perfused hearts triggered a redistribution in phosphotransfer flux with significant increase in creatine kinase and glycolytic rates. High energy phosphoryl fluxes through creatine kinase, adenylate kinase, and glycolysis in preconditioned hearts correlated tightly with post-ischemic functional recovery. This was associated with enhanced metabolite exchange between subcellular compartments, manifested by augmented transfer of inorganic phosphate from cellular ATPases to mitochondrial ATP synthase. Preconditioning-induced energetic remodeling protected cellular ATP synthesis and ATP consumption, improving contractile performance following ischemia-reperfusion insult. Thus, the plasticity of phosphotransfer networks contributes to the effective functioning of the cellular energetic system, providing a mechanism for increased tolerance toward injury.

Original languageEnglish (US)
Pages (from-to)44812-44819
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number48
DOIs
StatePublished - Nov 30 2001

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Adenosine Triphosphate
Nuclear magnetic resonance
Creatine Kinase
Mitochondrial Proton-Translocating ATPases
Fluxes
Adenylate Kinase
Ischemic Preconditioning
Wounds and Injuries
Glycolysis
Metabolites
Energy Metabolism
Nuclear magnetic resonance spectroscopy
Reperfusion
Plasticity
Adenosine Triphosphatases
Cell Survival
Magnetic Resonance Spectroscopy
Ischemia
Phosphates
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cellular energetics in the preconditioned state : Protective role for phosphotransfer reactions captured by 18O-assisted 31P NMR. / Pucar, Darko; Dzeja, Petras P.; Bast, Peter; Juranic, Nenad; Macura, Slobodan; Terzic, Andre.

In: Journal of Biological Chemistry, Vol. 276, No. 48, 30.11.2001, p. 44812-44819.

Research output: Contribution to journalArticle

Pucar, Darko ; Dzeja, Petras P. ; Bast, Peter ; Juranic, Nenad ; Macura, Slobodan ; Terzic, Andre. / Cellular energetics in the preconditioned state : Protective role for phosphotransfer reactions captured by 18O-assisted 31P NMR. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 48. pp. 44812-44819.
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