Centella asiatica attenuates the neurobehavioral, neurochemical and histological changes in transient focal middle cerebral artery occlusion rats

Fakhrul Islam, Rizwana Tabassum, Kumar Vaibhav, Pallavi Shrivastava, Andleeb Khan, Md Ejaz Ahmed, Hayate Javed, Farah Islam, Sayeed Ahmad, M. Saeed Siddiqui, Mohammed M. Safhi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Centella asiatica has been used as psychoactive and antioxidant herbal medicine since ancient time. The present study was design to evaluate the preventive role of ethanolic extract of C. asiatica in middle cerebral artery occlusion (MCAO) in rats. Male Wistar rats were gavaged orally with C. asiatica extract (100, 200 and 300 mg/kg body weight once daily) for 21 days and thereafter subjected to right MCAO for 2 h followed by 22-h reperfusion. Brain injury was evaluated by 2,3,5-triphenyltetrazolium chloride and hematoxylin and eosin staining. Behavioural outcomes as neurological deficit, rota rod test, and grip strength were assessed. In addition, lipid peroxidation, enzymatic and non enzymatic antioxidants were analyzed to assess the oxidative stress. Our results revealed that C. asiatica administration greatly improved neurobehavioral activity and diminished infarction volume along with the restored histological morphology of brain in MCAO rats. Furthermore, supplementation with this extract to MCAO group has reduced the level of thiobarbituric acid reactive species, restored glutathione content and augmented the activities of antioxidant enzymes - catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase and superoxide dismutase in a dose-dependent manner in ischemic rats. The remarkable antioxidant activity of C. asiatica may be attributed to its bioactive triterpenes, asiatic acid, asiaticoside, madecassic acid and madecosside and may be translated to clinical level for prevention of ischemic stroke.

Original languageEnglish (US)
Pages (from-to)925-933
Number of pages9
JournalNeurological Sciences
Volume34
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Centella
Middle Cerebral Artery Infarction
Antioxidants
Triterpenes
Glutathione Reductase
Herbal Medicine
Hand Strength
Hematoxylin
Eosine Yellowish-(YS)
Glutathione Peroxidase
Glutathione Transferase
Catalase
Brain Injuries
Infarction
Lipid Peroxidation
Superoxide Dismutase
Reperfusion
Glutathione
Wistar Rats
Oxidative Stress

Keywords

  • Asiatic acid
  • Centella asiatica
  • Ischemia-reperfusion
  • Neurobehavior
  • Oxidative stress

ASJC Scopus subject areas

  • Dermatology
  • Clinical Neurology
  • Psychiatry and Mental health

Cite this

Centella asiatica attenuates the neurobehavioral, neurochemical and histological changes in transient focal middle cerebral artery occlusion rats. / Islam, Fakhrul; Tabassum, Rizwana; Vaibhav, Kumar; Shrivastava, Pallavi; Khan, Andleeb; Ejaz Ahmed, Md; Javed, Hayate; Islam, Farah; Ahmad, Sayeed; Saeed Siddiqui, M.; Safhi, Mohammed M.

In: Neurological Sciences, Vol. 34, No. 6, 01.06.2013, p. 925-933.

Research output: Contribution to journalArticle

Islam, F, Tabassum, R, Vaibhav, K, Shrivastava, P, Khan, A, Ejaz Ahmed, M, Javed, H, Islam, F, Ahmad, S, Saeed Siddiqui, M & Safhi, MM 2013, 'Centella asiatica attenuates the neurobehavioral, neurochemical and histological changes in transient focal middle cerebral artery occlusion rats', Neurological Sciences, vol. 34, no. 6, pp. 925-933. https://doi.org/10.1007/s10072-012-1163-1
Islam, Fakhrul ; Tabassum, Rizwana ; Vaibhav, Kumar ; Shrivastava, Pallavi ; Khan, Andleeb ; Ejaz Ahmed, Md ; Javed, Hayate ; Islam, Farah ; Ahmad, Sayeed ; Saeed Siddiqui, M. ; Safhi, Mohammed M. / Centella asiatica attenuates the neurobehavioral, neurochemical and histological changes in transient focal middle cerebral artery occlusion rats. In: Neurological Sciences. 2013 ; Vol. 34, No. 6. pp. 925-933.
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AU - Shrivastava, Pallavi

AU - Khan, Andleeb

AU - Ejaz Ahmed, Md

AU - Javed, Hayate

AU - Islam, Farah

AU - Ahmad, Sayeed

AU - Saeed Siddiqui, M.

AU - Safhi, Mohammed M.

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AB - Centella asiatica has been used as psychoactive and antioxidant herbal medicine since ancient time. The present study was design to evaluate the preventive role of ethanolic extract of C. asiatica in middle cerebral artery occlusion (MCAO) in rats. Male Wistar rats were gavaged orally with C. asiatica extract (100, 200 and 300 mg/kg body weight once daily) for 21 days and thereafter subjected to right MCAO for 2 h followed by 22-h reperfusion. Brain injury was evaluated by 2,3,5-triphenyltetrazolium chloride and hematoxylin and eosin staining. Behavioural outcomes as neurological deficit, rota rod test, and grip strength were assessed. In addition, lipid peroxidation, enzymatic and non enzymatic antioxidants were analyzed to assess the oxidative stress. Our results revealed that C. asiatica administration greatly improved neurobehavioral activity and diminished infarction volume along with the restored histological morphology of brain in MCAO rats. Furthermore, supplementation with this extract to MCAO group has reduced the level of thiobarbituric acid reactive species, restored glutathione content and augmented the activities of antioxidant enzymes - catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase and superoxide dismutase in a dose-dependent manner in ischemic rats. The remarkable antioxidant activity of C. asiatica may be attributed to its bioactive triterpenes, asiatic acid, asiaticoside, madecassic acid and madecosside and may be translated to clinical level for prevention of ischemic stroke.

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