Central leptin versus ghrelin

Effects on bone marrow adiposity and gene expression

Suresh Ambati, Qiang Li, Srujana Rayalam, Diane L. Hartzell, Mary Anne Della-Fera, Mark W Hamrick, Clifton A. Baile

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study compared the central effects of ghrelin and leptin on body and bone marrow adiposity and gene expression in adipose tissue and bone marrow. Male Sprague-Dawley rats were injected intracerebroventricular (ICV) twice daily with control, 66 ng ghrelin (G66), 330 ng ghrelin (G330), or 5 μg leptin (L5) for 5 days. Food intake (FI) and body weight (BW) were measured daily. Gene expression in adipose tissue and bone marrow was assessed using RT-PCR. Leptin reduced FI (P < 0.05) and BW (P < 0.05), whereas ghrelin increased BW (P < 0.05) without affecting FI. Leptin decreased fat pad weights, whereas ghrelin (G330) increased fat pad weights (P < 0.05). In epididymal adipose tissue, leptin increased expression of lipolysis marker ADRB2 and thermogenesis marker MFN2 and decreased expression of adipogenic markers, FASN, SLC2A4, and SCD1, whereas ghrelin increased expression of FASN and SCD1. Leptin decreased bone marrow adipocyte size and number; however, ghrelin had no effect on these parameters. In whole bone marrow, leptin decreased expression of FASN and SCD1 and increased expression of DLK1, whereas ghrelin (G330) decreased expression of COL1A1. Thus, leptin induces similar changes in bone marrow and adipose tissue gene expression, reflecting the decreased adiposity in both compartments.

Original languageEnglish (US)
Pages (from-to)115-123
Number of pages9
JournalEndocrine
Volume37
Issue number1
DOIs
StatePublished - Feb 1 2010

Fingerprint

Ghrelin
Adiposity
Leptin
Bone Marrow
Gene Expression
Adipose Tissue
Eating
Body Weight
Weights and Measures
Thermogenesis
Lipolysis
Adipocytes
Sprague Dawley Rats
Bone and Bones
Polymerase Chain Reaction

Keywords

  • Body weight
  • Bone marrow
  • Food intake
  • Gene expression

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Ambati, S., Li, Q., Rayalam, S., Hartzell, D. L., Della-Fera, M. A., Hamrick, M. W., & Baile, C. A. (2010). Central leptin versus ghrelin: Effects on bone marrow adiposity and gene expression. Endocrine, 37(1), 115-123. https://doi.org/10.1007/s12020-009-9274-z

Central leptin versus ghrelin : Effects on bone marrow adiposity and gene expression. / Ambati, Suresh; Li, Qiang; Rayalam, Srujana; Hartzell, Diane L.; Della-Fera, Mary Anne; Hamrick, Mark W; Baile, Clifton A.

In: Endocrine, Vol. 37, No. 1, 01.02.2010, p. 115-123.

Research output: Contribution to journalArticle

Ambati, S, Li, Q, Rayalam, S, Hartzell, DL, Della-Fera, MA, Hamrick, MW & Baile, CA 2010, 'Central leptin versus ghrelin: Effects on bone marrow adiposity and gene expression', Endocrine, vol. 37, no. 1, pp. 115-123. https://doi.org/10.1007/s12020-009-9274-z
Ambati, Suresh ; Li, Qiang ; Rayalam, Srujana ; Hartzell, Diane L. ; Della-Fera, Mary Anne ; Hamrick, Mark W ; Baile, Clifton A. / Central leptin versus ghrelin : Effects on bone marrow adiposity and gene expression. In: Endocrine. 2010 ; Vol. 37, No. 1. pp. 115-123.
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