Ceramide and S1P signaling in embryonic stem cell differentiation

Guanghu Wang, Stefka D. Spassieva, Erhard Bieberich

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term “morphogenetic lipids” for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-171
Number of pages19
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1697
ISSN (Print)1064-3745

Keywords

  • Apoptosis
  • Ceramide
  • Neuroprogenitor
  • Oligodendrocyte precursor
  • Sphingolipid
  • Sphingosine-1-phosphate
  • Teratoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, G., Spassieva, S. D., & Bieberich, E. (2018). Ceramide and S1P signaling in embryonic stem cell differentiation. In Methods in Molecular Biology (pp. 153-171). (Methods in Molecular Biology; Vol. 1697). Humana Press Inc.. https://doi.org/10.1007/7651_2017_43