Ceramide-induced vasorelaxation: An inhibitory action on protein kinase C

Douglas G. Johns, Jong Shiaw Jin, Dixon W. Wilde, R. Clinton Webb

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Experiments were designed to examine the role of sphingosine, PP2A phosphatases, and protein kinase C (PKC) inhibition in mediating the vasodilatory effects of ceramide in rat thoracic aorta. Sphingosine did not cause vasorelaxation, and oleoylethanolamine, a ceramidase inhibitor, did not affect sphingomyelinase-induced relaxation. Okadaic acid potentiated the relaxation response to ceramide. These observations rule out involvement of sphingosine and PP2A phosphatases in mediating ceramide-induced relaxation. Sphingomyelinase attenuated contractile and single-cell intracellular calcium responses to phorbol ester. Chelerythrine incubation potentiated the relaxation response to ceramide. These observations support a role for PKC inhibition in mediating the vasodilatory effects of ceramide. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)415-421
Number of pages7
JournalGeneral Pharmacology
Volume33
Issue number5
DOIs
StatePublished - Nov 1 1999
Externally publishedYes

Keywords

  • Ceramide
  • Okadaic acid
  • Oleoylethanolamine
  • Sphingosine
  • Vascular smooth muscle
  • Vasorelaxation

ASJC Scopus subject areas

  • Pharmacology

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