Cerebral Intraventricular 6-Hydroxydopamine Prevents Vascular Changes in the Mineralocorticoid Hypertensive Rat

T. E. Mecca, F. S. Lamb, J. L. Hall, R Clinton Webb

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Abstract

The effect of cerebral intraventricular administration of 6-hydroxydopamine (6-OHDA) on blood pressure and vascular smooth muscle responsiveness in deoxycorticosterone acetate (DOCA)-treated rats was assessed. Rats treated with 6-OHDA and DOCA had significantly lower systolic blood pressures (142 ± 8 mm Hg) than rats treated with DOCA alone (185 ± 5 mm Hg). After 5 weeks of DOCA treatment, femoral arteries and aortae were excised from these rats, cut helically into strips, and placed in a muscle bath to record isometric force. Dose-response curves to serotonin were shifted to the left in femoral arteries from DOCA-treated rats compared to both control and 6-OHDA-DOCA-treated rats (ED50: DOCA = 6.8 × 10−8 M, control = 27.9 × 10−8 M, 6-OHDA-DOCA = 13.4 × 10−8 M). Arachidonic acid, the prostaglandin precursor, produced greater maximal contractions in femoral artery strips of DOCA-treated rats (358 ± 56 mg) than in those from controls (115 ± 31 mg). The maximal response to arachidonic acid in arteries from 6-OHDA-DOCA rats (203 ± 78 mg) was not different from control values. Ouabain produced a greater maximal response in aortic strips from DOCA rats (658 ± 165 mg) compared to those from control (196 ± 72 mg) or 6-OHDA-DOCA (309 ± 87 mg) rats. We conclude that increased vascular responsiveness to serotonin, arachidonic acid, and ouabain in DOCA hypertensive rats is secondary to a central action of the mineralocorticoid.

Original languageEnglish (US)
Pages (from-to)248-253
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume179
Issue number2
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

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Desoxycorticosterone
Mineralocorticoids
Oxidopamine
Blood Vessels
Rats
Acetates
Femoral Artery
Arachidonic Acid
Blood pressure
Ouabain
Blood Pressure
Muscle
Serotonin
Baths
Vascular Smooth Muscle
Prostaglandins
Aorta

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cerebral Intraventricular 6-Hydroxydopamine Prevents Vascular Changes in the Mineralocorticoid Hypertensive Rat. / Mecca, T. E.; Lamb, F. S.; Hall, J. L.; Webb, R Clinton.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 179, No. 2, 01.01.1985, p. 248-253.

Research output: Contribution to journalArticle

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