Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans

Merlin Manavathu, Suresh Gunasekaran, Quallyna Porte, Elias Manavathu, Muthukumaran Gunasekaran

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Candida albicans is a dimorphic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The intracellular level of glutathione, which helps to maintain the redox potential of the cell, is decreased significantly during the thermal induction of yeast-to-mycelium conversion. The reason for the decline of glutathione in the mycelial form is not understood. We have, therefore, investigated the levels of glutathione reductase, glutathione S-transferase, γ-glutamyltranspeptidase, and glutathione peroxidase, four key enzymes involved in glutathione metabolism, in the yeast and mycelial forms. Yeast cells of C. albicans 3153A were induced in Lee's medium (pH 6.5) at 37°C for 3 h to produce germ tubes. Cell lysates were prepared from yeast and mycelial cells, and glutathione reductase, glutathione S-transferase, γ-glutamyltranspeptidase, and glutathione peroxidase were assayed spectrophotometrically. There was a 640% increase of the level of γ-glutamyltranspeptidase in the germ tubes as compared with the yeast cells. No other significant alteration of the levels of enzymes was noted. This increased activity of γ-glutamyltranspeptidase, which cleaves the glutamic acid residue of glutathione (Glu-Cys-Gly) appears to be, at least in part, responsible for the rapid decrease of the intracellular glutathione in C. albicans during the yeast-to-mycelium conversion.

Original languageEnglish (US)
Pages (from-to)76-79
Number of pages4
JournalCanadian Journal of Microbiology
Volume42
Issue number1
DOIs
StatePublished - Jan 1 1996

Fingerprint

Mycelium
Candida albicans
Glutathione
Yeasts
Enzymes
Glutathione Reductase
Glutathione Peroxidase
Glutathione Transferase
Oxidation-Reduction
Glutamic Acid
Hot Temperature

Keywords

  • Candica albicans
  • Dimorphism
  • Glutathione
  • Glutathione peroxidase
  • Glutathione reductase
  • γ-Glutamyltranspeptidase

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans. / Manavathu, Merlin; Gunasekaran, Suresh; Porte, Quallyna; Manavathu, Elias; Gunasekaran, Muthukumaran.

In: Canadian Journal of Microbiology, Vol. 42, No. 1, 01.01.1996, p. 76-79.

Research output: Contribution to journalArticle

Manavathu, M, Gunasekaran, S, Porte, Q, Manavathu, E & Gunasekaran, M 1996, 'Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans', Canadian Journal of Microbiology, vol. 42, no. 1, pp. 76-79. https://doi.org/10.1139/m96-011
Manavathu, Merlin ; Gunasekaran, Suresh ; Porte, Quallyna ; Manavathu, Elias ; Gunasekaran, Muthukumaran. / Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans. In: Canadian Journal of Microbiology. 1996 ; Vol. 42, No. 1. pp. 76-79.
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