Changes in stiffness of resin-infiltrated demineralized dentin after remineralization by a bottom-up biomimetic approach

Li sha Gu, Bradford P. Huffman, Dwayne D. Arola, Young Kyung Kim, Sui Mai, Mohammed Elsayed Elsalanty, Jun qi Ling, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This study examined changes in elastic modulus, mineral density and ultrastructure of resin-infiltrated dentin after biomimetic remineralization. Sixty demineralized dentin beams were infiltrated with Clearfil Tri-S Bond, One-Step or Prime&Bond NT. They were immersed in simulated body fluid (SBF) for 1 week to maximize water sorption before determining the baseline elastic moduli. For each adhesive (N = 20) half of the beams remained immersed in SBF (control). The rest were immersed in a biomimetic remineralization medium. The elastic moduli were measured weekly for 15 additional weeks. Representative remineralized specimens were evaluated by X-ray microtomography and transmission electron microscopy (TEM). The elastic moduli of control resin-infiltrated dentin remained consistently low, while those immersed in the biomimetic remineralization medium increased by 55-118% after 4 months. X-ray microtomography of the remineralized specimens revealed decreases in mineral density from the beam surface to the beam core that were indicative of external mineral aggregation and internal mineral deposition. Interfibrillar and intrafibrillar remineralization of resin-sparse intertubular dentin were seen under TEM, together with remineralized peritubular dentin. Biomimetic remineralization occurs by diffusion of nanoprecursors and biomimetic analogs in completely demineralized resin-infiltrated dentin and proceeds without the contribution of materials released from a mineralized dentin base.

Original languageEnglish (US)
Pages (from-to)1453-1461
Number of pages9
JournalActa biomaterialia
Volume6
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Biomimetics
Dentin
Resins
Stiffness
Minerals
Elastic Modulus
Elastic moduli
Body fluids
X-Ray Microtomography
Body Fluids
Transmission Electron Microscopy
Transmission electron microscopy
X rays
Sorption
Adhesives
Agglomeration
Water

Keywords

  • Bio-remineralization
  • Elastic modulus
  • Intrafibrillar remineralization
  • Macro-hybrid layer
  • Peritubular dentin

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Changes in stiffness of resin-infiltrated demineralized dentin after remineralization by a bottom-up biomimetic approach. / Gu, Li sha; Huffman, Bradford P.; Arola, Dwayne D.; Kim, Young Kyung; Mai, Sui; Elsalanty, Mohammed Elsayed; Ling, Jun qi; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Acta biomaterialia, Vol. 6, No. 4, 01.04.2010, p. 1453-1461.

Research output: Contribution to journalArticle

Gu, Li sha ; Huffman, Bradford P. ; Arola, Dwayne D. ; Kim, Young Kyung ; Mai, Sui ; Elsalanty, Mohammed Elsayed ; Ling, Jun qi ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Changes in stiffness of resin-infiltrated demineralized dentin after remineralization by a bottom-up biomimetic approach. In: Acta biomaterialia. 2010 ; Vol. 6, No. 4. pp. 1453-1461.
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