Chapter 11 Intravital Microscopic Investigation of Leukocyte Interactions with the Blood Vessel Wall

Klaus Ley, Javier Mestas, Maria K. Pospieszalska, Prithu Sundd, Alexander Groisman, Alexander Zarbock

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Intravital microscopy is a method to study the microcirculation in living tissues. Transillumination, oblique reflected light illumination, continuous and stroboscopic epifluorescence microscopy can be used to visualized specific cells and molecules. Intravital microscopy is further enhanced by the advent of laser scanning.spinning disk confocal and multi-photon microscopy. Recent advances include blood-perfused flow chambers and microfluidic devises for the study of blood cell interactions with molecularly defined substrates. This chapter focuses on the application of these techniques to study leukocyte interactions with the vascular wall and molecular surfaces.

Original languageEnglish (US)
Title of host publicationAngiogenesis
Subtitle of host publicationIn Vivo Systems, Part B
EditorsDavid Cheresh
Pages255-279
Number of pages25
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume445
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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