Characteristics of the genome of Arsenophonus nasoniae, son-killer bacterium of the wasp Nasonia

A. C. Darby, Jeong-Hyeon Choi, T. Wilkes, M. A. Hughes, J. H. Werren, G. D.D. Hurst, J. K. Colbourne

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We report the properties of a draft genome sequence of the bacterium Arsenophonus nasoniae, son-killer bacterium of Nasonia vitripennis. The genome sequence data from this study are the first for a male-killing bacterium, and represent a microorganism that is unusual compared with other sequenced symbionts, in having routine vertical and horizontal transmission, two alternating hosts, and being culturable on cell-free media. The resulting sequence totals c. 3.5 Mbp and is annotated to contain 3332 predicted open reading frames (ORFs). Therefore, Arsenophonus represents a relatively large genome for an insect symbiont. The annotated ORF set suggests that the microbe is capable of a broad array of metabolic functions, well beyond those found for reproductive parasite genomes sequenced to date and more akin to horizontally transmitted and secondary symbionts. We also find evidence of genetic transfer from Wolbachia symbionts, and phage exchange with other gammaproteobacterial symbionts. These findings reflect the complex biology of a bacterium that is able to live, invade and survive multiple host environments while resisting immune responses.

Original languageEnglish (US)
Pages (from-to)75-89
Number of pages15
JournalInsect Molecular Biology
Volume19
Issue numberSUPPL. 1
DOIs
StatePublished - Feb 1 2010

Fingerprint

Arsenophonus nasoniae
Nasonia
Wasps
symbionts
Genome
Bacteria
genome
bacteria
Open Reading Frames
Insect Genome
Wolbachia
open reading frames
Arsenophonus
Nasonia vitripennis
microorganisms
Bacteriophages
Parasites
bacteriophages
immune response
parasites

Keywords

  • Arsenophonus
  • Insect symbiont
  • Male-killing
  • Microbial genome sequence
  • Photorhabdus
  • Toxins
  • Wolbachia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Darby, A. C., Choi, J-H., Wilkes, T., Hughes, M. A., Werren, J. H., Hurst, G. D. D., & Colbourne, J. K. (2010). Characteristics of the genome of Arsenophonus nasoniae, son-killer bacterium of the wasp Nasonia. Insect Molecular Biology, 19(SUPPL. 1), 75-89. https://doi.org/10.1111/j.1365-2583.2009.00950.x

Characteristics of the genome of Arsenophonus nasoniae, son-killer bacterium of the wasp Nasonia. / Darby, A. C.; Choi, Jeong-Hyeon; Wilkes, T.; Hughes, M. A.; Werren, J. H.; Hurst, G. D.D.; Colbourne, J. K.

In: Insect Molecular Biology, Vol. 19, No. SUPPL. 1, 01.02.2010, p. 75-89.

Research output: Contribution to journalArticle

Darby, A. C. ; Choi, Jeong-Hyeon ; Wilkes, T. ; Hughes, M. A. ; Werren, J. H. ; Hurst, G. D.D. ; Colbourne, J. K. / Characteristics of the genome of Arsenophonus nasoniae, son-killer bacterium of the wasp Nasonia. In: Insect Molecular Biology. 2010 ; Vol. 19, No. SUPPL. 1. pp. 75-89.
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