Characterization and phospholipase D mediation of the angiotensin II priming response in adrenal glomerulosa cells

Wendy B Bollag, Patricia Kent, Stephanie White, Mariya Malinova, Carlos M Isales, Roberto A. Calle

Research output: Contribution to journalArticle

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Abstract

Bovine adrenal glomerulosa cells are primed by an initial treatment with angiotensin II (AngII) to respond with enhanced secretion to a second exposure to AngII or agents that increase calcium influx. We hypothesized that the mechanism of priming involves a persistent increase in diacylglycerol (DAG) generated via sustained activity of phospholipase D (PLD). In this report, we sought to define the time frame of this priming response as well as determine its mechanism using assays of aldosterone secretion, PLD activation, and radiolabeled diacylglycerol levels. We found that in primary cultures priming was observed for up to 50 min after AngII washout, suggesting that the priming window is protracted in these cultures relative to freshly isolated cells. The phorbol ester, phorbol 12,13-dibutyrate (PDBu), was used to investigate the role of sustained PLD activation in the persistent DAG and priming responses. PDBu was able to both prime glomerulosa cells to respond with enhanced secretion to AngII and elicit a persistent increase in DAG after PDBu washout. This persistent increase in DAG levels with an initial exposure to PDBu or AngII was not the result of maintained PLD activity after agent removal because PLD activation returned to basal levels by 30 min after washout. Finally, inhibition of PLD signaling during the initial AngII treatment inhibited the subsequent response to AngII or another agent that increases calcium influx. Thus, our results suggest that persistent DAG resulting from PLD signaling mediates the priming response to AngII or PDBu.

Original languageEnglish (US)
Pages (from-to)585-593
Number of pages9
JournalEndocrinology
Volume148
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Zona Glomerulosa
Phospholipase D
Angiotensin II
Phorbol 12,13-Dibutyrate
Diglycerides
Calcium
Phorbol Esters
Aldosterone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Characterization and phospholipase D mediation of the angiotensin II priming response in adrenal glomerulosa cells. / Bollag, Wendy B; Kent, Patricia; White, Stephanie; Malinova, Mariya; Isales, Carlos M; Calle, Roberto A.

In: Endocrinology, Vol. 148, No. 2, 01.02.2007, p. 585-593.

Research output: Contribution to journalArticle

Bollag, Wendy B ; Kent, Patricia ; White, Stephanie ; Malinova, Mariya ; Isales, Carlos M ; Calle, Roberto A. / Characterization and phospholipase D mediation of the angiotensin II priming response in adrenal glomerulosa cells. In: Endocrinology. 2007 ; Vol. 148, No. 2. pp. 585-593.
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