Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3

S. Ren, T. Ariga, J. N. Scarsdale, Y. Zhang, A. Slominski, P. O. Livingston, G. Ritter, Y. Kushi, Robert K Yu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We previously reported a hamster animal model of melanoma in which the tumor tissue expresses gangliosides GM3, GD3, and O-acetyl GD3. This ganglioside pattern is similar to that in human melanomas (Ren, S., A. Slominski, and R. K. Yu. 1989 Cancer Res. 49: 7051). In this study, we isolated and purified these gangliosides using chloroform-methanol extraction, Folch partition, chromatographies on DEAE-Sephadex A-25, and Iatrobeads columns. The yields of gangliosides GM3, GD3, and O-acetyl GD3 were 44.1 mg, 19.6 mg, and 9 mg per 100 g of Ma melanotic melanoma tissues, respectively. The structures of these gangliosides were characterized by periodate oxidation, gas chromatographic (GC) analysis, fast-atom bombardment-mass spectrometry (FAB-MS), and nuclear magnetic resonance (NMR) studies. The structure of hamster melanoma O-acetyl GD3 is different from the 9-O-acetyl GD3 previously reported in human melanoma. The major fatty acids of this ganglioside are C16:0, C18:0, C20:0, C22:0, and C24:0 and the longchain base is C18-sphingosine.

Original languageEnglish (US)
Pages (from-to)1565-1572
Number of pages8
JournalJournal of Lipid Research
Volume34
Issue number9
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Gangliosides
Cricetinae
Melanoma
G(M3) Ganglioside
Tissue
Sphingosine
Fast Atom Bombardment Mass Spectrometry
Chloroform
Chromatography
Gas chromatography
Mass spectrometry
Methanol
Tumors
Animals
Fatty Acids
Gas Chromatography
Nuclear magnetic resonance
Neoplasms
Magnetic Resonance Spectroscopy
Atoms

Keywords

  • 7-O-acetyl GD3
  • fast-atom bombardment-mass spectrometry
  • nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Ren, S., Ariga, T., Scarsdale, J. N., Zhang, Y., Slominski, A., Livingston, P. O., ... Yu, R. K. (1993). Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3. Journal of Lipid Research, 34(9), 1565-1572.

Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3. / Ren, S.; Ariga, T.; Scarsdale, J. N.; Zhang, Y.; Slominski, A.; Livingston, P. O.; Ritter, G.; Kushi, Y.; Yu, Robert K.

In: Journal of Lipid Research, Vol. 34, No. 9, 01.01.1993, p. 1565-1572.

Research output: Contribution to journalArticle

Ren, S, Ariga, T, Scarsdale, JN, Zhang, Y, Slominski, A, Livingston, PO, Ritter, G, Kushi, Y & Yu, RK 1993, 'Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3', Journal of Lipid Research, vol. 34, no. 9, pp. 1565-1572.
Ren S, Ariga T, Scarsdale JN, Zhang Y, Slominski A, Livingston PO et al. Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3. Journal of Lipid Research. 1993 Jan 1;34(9):1565-1572.
Ren, S. ; Ariga, T. ; Scarsdale, J. N. ; Zhang, Y. ; Slominski, A. ; Livingston, P. O. ; Ritter, G. ; Kushi, Y. ; Yu, Robert K. / Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3. In: Journal of Lipid Research. 1993 ; Vol. 34, No. 9. pp. 1565-1572.
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