Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3

S. Ren, T. Ariga, J. N. Scarsdale, Y. Zhang, A. Slominski, P. O. Livingston, G. Ritter, Y. Kushi, R. K. Yu

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We previously reported a hamster animal model of melanoma in which the tumor tissue expresses gangliosides GM3, GD3, and O-acetyl GD3. This ganglioside pattern is similar to that in human melanomas (Ren, S., A. Slominski, and R. K. Yu. 1989 Cancer Res. 49: 7051). In this study, we isolated and purified these gangliosides using chloroform-methanol extraction, Folch partition, chromatographies on DEAE-Sephadex A-25, and Iatrobeads columns. The yields of gangliosides GM3, GD3, and O-acetyl GD3 were 44.1 mg, 19.6 mg, and 9 mg per 100 g of Ma melanotic melanoma tissues, respectively. The structures of these gangliosides were characterized by periodate oxidation, gas chromatographic (GC) analysis, fast-atom bombardment-mass spectrometry (FAB-MS), and nuclear magnetic resonance (NMR) studies. The structure of hamster melanoma O-acetyl GD3 is different from the 9-O-acetyl GD3 previously reported in human melanoma. The major fatty acids of this ganglioside are C16:0, C18:0, C20:0, C22:0, and C24:0 and the longchain base is C18-sphingosine.

Original languageEnglish (US)
Pages (from-to)1565-1572
Number of pages8
JournalJournal of Lipid Research
Volume34
Issue number9
StatePublished - 1993
Externally publishedYes

Keywords

  • 7-O-acetyl GD3
  • fast-atom bombardment-mass spectrometry
  • nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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    Ren, S., Ariga, T., Scarsdale, J. N., Zhang, Y., Slominski, A., Livingston, P. O., Ritter, G., Kushi, Y., & Yu, R. K. (1993). Characterization of a hamster melanoma-associated ganglioside antigen as 7-O-acetylated disialoganglioside GD3. Journal of Lipid Research, 34(9), 1565-1572.