Abstract
Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5% and 2.5% of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 723-739 |
| Number of pages | 17 |
| Journal | Hemoglobin |
| Volume | 12 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - Jan 1 1988 |
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ASJC Scopus subject areas
- Hematology
- Genetics(clinical)
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Characterization of abnormalities in the γglobin gene arrangements of japanese newborns. / Harano, T.; Harano, K.; Ukita, H.; Wada, Y.; Hayashi, A.; Ohba, Y.; Miyaji, T.; Kutlar, Ferdane; Huisman, T. H.J.
In: Hemoglobin, Vol. 12, No. 5-6, 01.01.1988, p. 723-739.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of abnormalities in the γglobin gene arrangements of japanese newborns
AU - Harano, T.
AU - Harano, K.
AU - Ukita, H.
AU - Wada, Y.
AU - Hayashi, A.
AU - Ohba, Y.
AU - Miyaji, T.
AU - Kutlar, Ferdane
AU - Huisman, T. H.J.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5% and 2.5% of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.
AB - Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5% and 2.5% of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.
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U2 - 10.3109/03630268808991666
DO - 10.3109/03630268808991666
M3 - Article
VL - 12
SP - 723
EP - 739
JO - Hemoglobin
JF - Hemoglobin
SN - 0363-0269
IS - 5-6
ER -