Characterization of abnormalities in the γglobin gene arrangements of japanese newborns

T. Harano, K. Harano, H. Ukita, Y. Wada, A. Hayashi, Y. Ohba, T. Miyaji, Ferdane Kutlar, T. H.J. Huisman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5% and 2.5% of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.

Original languageEnglish (US)
Pages (from-to)723-739
Number of pages17
JournalHemoglobin
Volume12
Issue number5-6
DOIs
StatePublished - Jan 1 1988

Fingerprint

Gene Order
Globins
Genes
Newborn Infant
Chromosomes
Haplotypes
Genotype
Fetal Blood
Islands
Gene Frequency
Japan
Polymorphism
Gene Expression
Blood
DNA

ASJC Scopus subject areas

  • Hematology
  • Genetics(clinical)
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Harano, T., Harano, K., Ukita, H., Wada, Y., Hayashi, A., Ohba, Y., ... Huisman, T. H. J. (1988). Characterization of abnormalities in the γglobin gene arrangements of japanese newborns. Hemoglobin, 12(5-6), 723-739. https://doi.org/10.3109/03630268808991666

Characterization of abnormalities in the γglobin gene arrangements of japanese newborns. / Harano, T.; Harano, K.; Ukita, H.; Wada, Y.; Hayashi, A.; Ohba, Y.; Miyaji, T.; Kutlar, Ferdane; Huisman, T. H.J.

In: Hemoglobin, Vol. 12, No. 5-6, 01.01.1988, p. 723-739.

Research output: Contribution to journalArticle

Harano, T, Harano, K, Ukita, H, Wada, Y, Hayashi, A, Ohba, Y, Miyaji, T, Kutlar, F & Huisman, THJ 1988, 'Characterization of abnormalities in the γglobin gene arrangements of japanese newborns', Hemoglobin, vol. 12, no. 5-6, pp. 723-739. https://doi.org/10.3109/03630268808991666
Harano T, Harano K, Ukita H, Wada Y, Hayashi A, Ohba Y et al. Characterization of abnormalities in the γglobin gene arrangements of japanese newborns. Hemoglobin. 1988 Jan 1;12(5-6):723-739. https://doi.org/10.3109/03630268808991666
Harano, T. ; Harano, K. ; Ukita, H. ; Wada, Y. ; Hayashi, A. ; Ohba, Y. ; Miyaji, T. ; Kutlar, Ferdane ; Huisman, T. H.J. / Characterization of abnormalities in the γglobin gene arrangements of japanese newborns. In: Hemoglobin. 1988 ; Vol. 12, No. 5-6. pp. 723-739.
@article{a6e353aab3d344b98ca287a027217203,
title = "Characterization of abnormalities in the γglobin gene arrangements of japanese newborns",
abstract = "Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5{\%} and 2.5{\%} of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.",
author = "T. Harano and K. Harano and H. Ukita and Y. Wada and A. Hayashi and Y. Ohba and T. Miyaji and Ferdane Kutlar and Huisman, {T. H.J.}",
year = "1988",
month = "1",
day = "1",
doi = "10.3109/03630268808991666",
language = "English (US)",
volume = "12",
pages = "723--739",
journal = "Hemoglobin",
issn = "0363-0269",
publisher = "Informa Healthcare",
number = "5-6",

}

TY - JOUR

T1 - Characterization of abnormalities in the γglobin gene arrangements of japanese newborns

AU - Harano, T.

AU - Harano, K.

AU - Ukita, H.

AU - Wada, Y.

AU - Hayashi, A.

AU - Ohba, Y.

AU - Miyaji, T.

AU - Kutlar, Ferdane

AU - Huisman, T. H.J.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5% and 2.5% of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.

AB - Cord blood samples from 889 healthy Japanese newborns from three districts of Honshu Island were studied with the purpose of characterizing the γglobin genes in the Japanese. The A γ T gene frequency was 0.159 which is the same as that found elsewhere in Japan. The haplotype of the chromosome with the found elsewhere A γ T gene was [-+ + - + (+ or -) (+ or -)] at eight polymorphic sites. Data from analyses of DNA and the proportion of γ chains in Hb F showed the existence of various kinds of γglobin gene arrangements; six genotypes were observed for individuals with high G γ values and triple or quadruple γglobin gene arrangements, and seven genotypes for babies with low G γ values and a single γglobin gene. The in vivo expression of the γglobin gene located at the third or fourth position in the multiple gene arrangement was found to be about 5% and 2.5% of the total, respectively. The haplotype for the chromosomes with a triple γglobin gene was [+ - (-) - +], but that for the chromosomes with a single γglobin gene remains unclear. In addition, a new type of Bgl II polymorphism in the G γ -globin gene was observed.

UR - http://www.scopus.com/inward/record.url?scp=0023745230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023745230&partnerID=8YFLogxK

U2 - 10.3109/03630268808991666

DO - 10.3109/03630268808991666

M3 - Article

VL - 12

SP - 723

EP - 739

JO - Hemoglobin

JF - Hemoglobin

SN - 0363-0269

IS - 5-6

ER -