Characterization of an antigen-antibody system associated with epidemic non-A,non-B hepatitis in west africa and experimental transmission of an infectious agent to primates

J. L. Sarthou, A. Budkowska, Madhav D Sharma, M. Lhuillier, J. Pillot

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

An epidemic of non-A, non-B hepatitis (NANBH) was studied in Tortiya, Ivory Coast, over a period of 17 months. Highly sensitive serologic tests performed on serum samples from patients with this type of hepatitis excluded both HAV (hepatitis A virus) and HBV (hepatitis B virus) as aetiologic agents. The mode of spread of the epidemic, clinical features and frequent cases of fulminant hepatitis in pregnant women resembled that of other recently described water-borne outbreaks of non-A,non-B hepatitis. Oral-faecal transmission of this form of NANBH was shown by experimental infection of African monkeys, which developed antibody of the IgM class specifically reacting with the antigen present in stools of infected persons. Using the immunoenzymatic test employing monkey IgM on solid phase and enzyme-labelled IgG purified from sera of patients convalescing from NANBH, we identified the antigen associated with the possible causative NANBH agent. These data confirm the existence of a distinct human NANBH agent responsible for epidemic out-breaks of NANBH. This agent is different from those of the postransfusion type of NANBH and antigenically unrelated to HAV. The direct serologic test presented here may be useful for diagnosis of an epidemic form of NANBH.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalAnnales de l'Institut Pasteur Virology
Volume137
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Hepatitis E
Western Africa
Primates
Hepatitis
Antigens
Antibodies
Hepatitis A virus
Serologic Tests
Haplorhini
Immunoglobulin M
Hepatitis C Antigens
Cote d'Ivoire
Immunoglobulin Isotypes
Serum
Hepatitis B virus
Disease Outbreaks
Pregnant Women
Immunoglobulin G
Water

Keywords

  • Antigen detection
  • Côted'Ivoire
  • Détection de l'antigène
  • ELISA
  • ELISA
  • Hépatite NANB
  • Ivory Coast
  • NANB hepatitis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{8c8ebf52a0414334b48ad39d3785f94f,
title = "Characterization of an antigen-antibody system associated with epidemic non-A,non-B hepatitis in west africa and experimental transmission of an infectious agent to primates",
abstract = "An epidemic of non-A, non-B hepatitis (NANBH) was studied in Tortiya, Ivory Coast, over a period of 17 months. Highly sensitive serologic tests performed on serum samples from patients with this type of hepatitis excluded both HAV (hepatitis A virus) and HBV (hepatitis B virus) as aetiologic agents. The mode of spread of the epidemic, clinical features and frequent cases of fulminant hepatitis in pregnant women resembled that of other recently described water-borne outbreaks of non-A,non-B hepatitis. Oral-faecal transmission of this form of NANBH was shown by experimental infection of African monkeys, which developed antibody of the IgM class specifically reacting with the antigen present in stools of infected persons. Using the immunoenzymatic test employing monkey IgM on solid phase and enzyme-labelled IgG purified from sera of patients convalescing from NANBH, we identified the antigen associated with the possible causative NANBH agent. These data confirm the existence of a distinct human NANBH agent responsible for epidemic out-breaks of NANBH. This agent is different from those of the postransfusion type of NANBH and antigenically unrelated to HAV. The direct serologic test presented here may be useful for diagnosis of an epidemic form of NANBH.",
keywords = "Antigen detection, C{\^o}ted'Ivoire, D{\'e}tection de l'antig{\`e}ne, ELISA, ELISA, H{\'e}patite NANB, Ivory Coast, NANB hepatitis",
author = "Sarthou, {J. L.} and A. Budkowska and Sharma, {Madhav D} and M. Lhuillier and J. Pillot",
year = "1986",
month = "1",
day = "1",
doi = "10.1016/S0769-2617(86)80208-8",
language = "English (US)",
volume = "137",
pages = "225--232",
journal = "Annales de l'Institut Pasteur Virology",
issn = "0769-2617",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Characterization of an antigen-antibody system associated with epidemic non-A,non-B hepatitis in west africa and experimental transmission of an infectious agent to primates

AU - Sarthou, J. L.

AU - Budkowska, A.

AU - Sharma, Madhav D

AU - Lhuillier, M.

AU - Pillot, J.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - An epidemic of non-A, non-B hepatitis (NANBH) was studied in Tortiya, Ivory Coast, over a period of 17 months. Highly sensitive serologic tests performed on serum samples from patients with this type of hepatitis excluded both HAV (hepatitis A virus) and HBV (hepatitis B virus) as aetiologic agents. The mode of spread of the epidemic, clinical features and frequent cases of fulminant hepatitis in pregnant women resembled that of other recently described water-borne outbreaks of non-A,non-B hepatitis. Oral-faecal transmission of this form of NANBH was shown by experimental infection of African monkeys, which developed antibody of the IgM class specifically reacting with the antigen present in stools of infected persons. Using the immunoenzymatic test employing monkey IgM on solid phase and enzyme-labelled IgG purified from sera of patients convalescing from NANBH, we identified the antigen associated with the possible causative NANBH agent. These data confirm the existence of a distinct human NANBH agent responsible for epidemic out-breaks of NANBH. This agent is different from those of the postransfusion type of NANBH and antigenically unrelated to HAV. The direct serologic test presented here may be useful for diagnosis of an epidemic form of NANBH.

AB - An epidemic of non-A, non-B hepatitis (NANBH) was studied in Tortiya, Ivory Coast, over a period of 17 months. Highly sensitive serologic tests performed on serum samples from patients with this type of hepatitis excluded both HAV (hepatitis A virus) and HBV (hepatitis B virus) as aetiologic agents. The mode of spread of the epidemic, clinical features and frequent cases of fulminant hepatitis in pregnant women resembled that of other recently described water-borne outbreaks of non-A,non-B hepatitis. Oral-faecal transmission of this form of NANBH was shown by experimental infection of African monkeys, which developed antibody of the IgM class specifically reacting with the antigen present in stools of infected persons. Using the immunoenzymatic test employing monkey IgM on solid phase and enzyme-labelled IgG purified from sera of patients convalescing from NANBH, we identified the antigen associated with the possible causative NANBH agent. These data confirm the existence of a distinct human NANBH agent responsible for epidemic out-breaks of NANBH. This agent is different from those of the postransfusion type of NANBH and antigenically unrelated to HAV. The direct serologic test presented here may be useful for diagnosis of an epidemic form of NANBH.

KW - Antigen detection

KW - Côted'Ivoire

KW - Détection de l'antigène

KW - ELISA

KW - ELISA

KW - Hépatite NANB

KW - Ivory Coast

KW - NANB hepatitis

UR - http://www.scopus.com/inward/record.url?scp=0022540464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022540464&partnerID=8YFLogxK

U2 - 10.1016/S0769-2617(86)80208-8

DO - 10.1016/S0769-2617(86)80208-8

M3 - Article

AN - SCOPUS:0022540464

VL - 137

SP - 225

EP - 232

JO - Annales de l'Institut Pasteur Virology

JF - Annales de l'Institut Pasteur Virology

SN - 0769-2617

ER -