Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung

Lesleyann Hawthorn, Leighton Stein, Jenniffer Panzarella, Gregory M. Loewen, Heinz Baumann

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Lung cancer accounts for 28% of all cancer deaths, a higher percentage than any other human cancer. Squamous Cell Carcinoma (SqCC) is the most common lung neoplasm and is a tumor that is extensively associated with tobacco use. Despite the association of many genetic alterations with lung cancer, the precise molecular mechanisms of tumorigenesis, for the most part, remain ambiguous. Although many studies of lung cancer have used global transcript profiling approaches designed to uncover genes or pathways that are important in lung tumorigenesis, no strong candidates have emerged. A lack of concurrence amongst these various studies can be attributed, in a large part, to the cellular heterogeneity within lung tissue. We have attempted to reduce this complication by designing a profiling strategy that will minimize the confounding involvement of tissue heterogeneity in gene expression of lung tumors. Specifically, we have profiled transcript expression levels in both isolated cells and tissues from SqCC and normal samples. Our strategy consists of combining and subtracting the input of these various cell types which has produced a unique transcript profile of the squamous carcinoma cell. We then analyzed the data using Pathways Assist analysis software to determine which processes may be involved in SqCC tumorigenesis. The MAP/ERK pathway involved in growth and differentiation was the pathway that was most frequently identified across all comparisons. In addition, biological interaction networks of the SqCC profile identified IL-8 as playing a potentially important role SqCC development.

Original languageEnglish (US)
Pages (from-to)129-142
Number of pages14
JournalLung Cancer
Volume53
Issue number2
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

Fingerprint

Squamous Cell Carcinoma
Lung
Lung Neoplasms
Carcinogenesis
Neoplasms
MAP Kinase Signaling System
Tobacco Use
Interleukin-8
Software
Gene Expression
Growth
Genes

Keywords

  • Expression profiles
  • Pathway analysis
  • Squamous cell carcinoma
  • Tissue heterogeneity

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

Cite this

Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung. / Hawthorn, Lesleyann; Stein, Leighton; Panzarella, Jenniffer; Loewen, Gregory M.; Baumann, Heinz.

In: Lung Cancer, Vol. 53, No. 2, 01.08.2006, p. 129-142.

Research output: Contribution to journalArticle

Hawthorn, Lesleyann ; Stein, Leighton ; Panzarella, Jenniffer ; Loewen, Gregory M. ; Baumann, Heinz. / Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung. In: Lung Cancer. 2006 ; Vol. 53, No. 2. pp. 129-142.
@article{01c02b5033a64981858f12466ac65055,
title = "Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung",
abstract = "Lung cancer accounts for 28{\%} of all cancer deaths, a higher percentage than any other human cancer. Squamous Cell Carcinoma (SqCC) is the most common lung neoplasm and is a tumor that is extensively associated with tobacco use. Despite the association of many genetic alterations with lung cancer, the precise molecular mechanisms of tumorigenesis, for the most part, remain ambiguous. Although many studies of lung cancer have used global transcript profiling approaches designed to uncover genes or pathways that are important in lung tumorigenesis, no strong candidates have emerged. A lack of concurrence amongst these various studies can be attributed, in a large part, to the cellular heterogeneity within lung tissue. We have attempted to reduce this complication by designing a profiling strategy that will minimize the confounding involvement of tissue heterogeneity in gene expression of lung tumors. Specifically, we have profiled transcript expression levels in both isolated cells and tissues from SqCC and normal samples. Our strategy consists of combining and subtracting the input of these various cell types which has produced a unique transcript profile of the squamous carcinoma cell. We then analyzed the data using Pathways Assist analysis software to determine which processes may be involved in SqCC tumorigenesis. The MAP/ERK pathway involved in growth and differentiation was the pathway that was most frequently identified across all comparisons. In addition, biological interaction networks of the SqCC profile identified IL-8 as playing a potentially important role SqCC development.",
keywords = "Expression profiles, Pathway analysis, Squamous cell carcinoma, Tissue heterogeneity",
author = "Lesleyann Hawthorn and Leighton Stein and Jenniffer Panzarella and Loewen, {Gregory M.} and Heinz Baumann",
year = "2006",
month = "8",
day = "1",
doi = "10.1016/j.lungcan.2006.04.015",
language = "English (US)",
volume = "53",
pages = "129--142",
journal = "Lung Cancer",
issn = "0169-5002",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung

AU - Hawthorn, Lesleyann

AU - Stein, Leighton

AU - Panzarella, Jenniffer

AU - Loewen, Gregory M.

AU - Baumann, Heinz

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Lung cancer accounts for 28% of all cancer deaths, a higher percentage than any other human cancer. Squamous Cell Carcinoma (SqCC) is the most common lung neoplasm and is a tumor that is extensively associated with tobacco use. Despite the association of many genetic alterations with lung cancer, the precise molecular mechanisms of tumorigenesis, for the most part, remain ambiguous. Although many studies of lung cancer have used global transcript profiling approaches designed to uncover genes or pathways that are important in lung tumorigenesis, no strong candidates have emerged. A lack of concurrence amongst these various studies can be attributed, in a large part, to the cellular heterogeneity within lung tissue. We have attempted to reduce this complication by designing a profiling strategy that will minimize the confounding involvement of tissue heterogeneity in gene expression of lung tumors. Specifically, we have profiled transcript expression levels in both isolated cells and tissues from SqCC and normal samples. Our strategy consists of combining and subtracting the input of these various cell types which has produced a unique transcript profile of the squamous carcinoma cell. We then analyzed the data using Pathways Assist analysis software to determine which processes may be involved in SqCC tumorigenesis. The MAP/ERK pathway involved in growth and differentiation was the pathway that was most frequently identified across all comparisons. In addition, biological interaction networks of the SqCC profile identified IL-8 as playing a potentially important role SqCC development.

AB - Lung cancer accounts for 28% of all cancer deaths, a higher percentage than any other human cancer. Squamous Cell Carcinoma (SqCC) is the most common lung neoplasm and is a tumor that is extensively associated with tobacco use. Despite the association of many genetic alterations with lung cancer, the precise molecular mechanisms of tumorigenesis, for the most part, remain ambiguous. Although many studies of lung cancer have used global transcript profiling approaches designed to uncover genes or pathways that are important in lung tumorigenesis, no strong candidates have emerged. A lack of concurrence amongst these various studies can be attributed, in a large part, to the cellular heterogeneity within lung tissue. We have attempted to reduce this complication by designing a profiling strategy that will minimize the confounding involvement of tissue heterogeneity in gene expression of lung tumors. Specifically, we have profiled transcript expression levels in both isolated cells and tissues from SqCC and normal samples. Our strategy consists of combining and subtracting the input of these various cell types which has produced a unique transcript profile of the squamous carcinoma cell. We then analyzed the data using Pathways Assist analysis software to determine which processes may be involved in SqCC tumorigenesis. The MAP/ERK pathway involved in growth and differentiation was the pathway that was most frequently identified across all comparisons. In addition, biological interaction networks of the SqCC profile identified IL-8 as playing a potentially important role SqCC development.

KW - Expression profiles

KW - Pathway analysis

KW - Squamous cell carcinoma

KW - Tissue heterogeneity

UR - http://www.scopus.com/inward/record.url?scp=33745622375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745622375&partnerID=8YFLogxK

U2 - 10.1016/j.lungcan.2006.04.015

DO - 10.1016/j.lungcan.2006.04.015

M3 - Article

VL - 53

SP - 129

EP - 142

JO - Lung Cancer

JF - Lung Cancer

SN - 0169-5002

IS - 2

ER -